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ZAVŘEL, Tomáš, Maria, A. SINETOVÁ, Diana BÚZOVÁ, Petra LITERÁKOVÁ and Jan ČERVENÝ. Characterization of a model cyanobacterium Synechocystis sp. PCC 6803 autotrophic growth in a flat-panel photobioreactor. ENGINEERING IN LIFE SCIENCES. WILEY-BLACKWELL, 2015, vol. 15, No 1, p. 122-132. ISSN 1618-0240. doi:10.1002/elsc.201300165.
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Basic information
Original name Characterization of a model cyanobacterium Synechocystis sp. PCC 6803 autotrophic growth in a flat-panel photobioreactor.
Authors ZAVŘEL, Tomáš (203 Czech Republic, belonging to the institution), Maria, A. SINETOVÁ (643 Russian Federation), Diana BÚZOVÁ (203 Czech Republic), Petra LITERÁKOVÁ (203 Czech Republic) and Jan ČERVENÝ (203 Czech Republic).
Other information
Original language English
Type of outcome Article in a journal
Field of Study Biotechnology and bionics
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.168
RIV identification code RIV/00216224:14310/15:00082477
Organization unit Faculty of Science
UT WoS 000347236300014
Keywords in English Carbon dioxide; Exponential phase; Growth optimization; Light; Temperature
Tags AKR, rivok
Tags International impact, Reviewed
Changed by Changed by: Ing. Andrea Mikešková, učo 137293. Changed: 25/4/2016 14:18.
We characterized the photoautotrophic growth of glucose-tolerant Synechocystis sp. PCC 6803 in a flat-panel photobioreactor running on a semicontinuous regime under various lights, temperatures, and influx carbon dioxide concentrations. The maximum reached growth rate was 0.135 h(-1), which corresponds to a doubling time of 5.13 ha growth speed never reported for Synechocystis before. Saturating red light intensity for the strain was 220-360 mol(photons)m(-2)s(-1), and we did not observe any photoinhibition up to 660 mol(photons)m(-2)s(-1). Synechocystis was able to grow under red light only; however, photons of wavelengths 405-585 and 670-700 nm further improved its growth. Optimal growth temperature was 35 degrees C. Below 32 degrees C, the growth rates decreased linearly with temperature coefficient (Q(10)) 1.70. Semicontinuous cultivation is known to be efficient for growth characterization and optimization. However, the assumption of correct growth rates calculationculture exponential growthis often not fulfilled. The semicontinuous setup in this study was operated as a turbidostat. Accurate online OD measurements with high time-resolution allowed fast and reliable growth rates determination. Repeating diluting frequencies (up to 18 dilutions per day) were essential for rapid growth stability evaluation. The presented setup provides improvement to previously published semicontinuous characterization strategies by decreasing experimental time requirements and maintaining the culture in exponential growth phase throughout the entire characterization procedure.
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