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@inproceedings{1230832, author = {Dobisová, Tereza and Hejátko, Jan}, address = {New York}, booktitle = {Plant Cell Morphogenesis: Methods and Protocols}, doi = {http://dx.doi.org/10.1007/978-1-62703-643-6_4}, editor = {Žárský V.}, keywords = {apical meristem; Arabidopsis thaliana; branching morphogenesis; cell communication; cell function; cell membrane; cell shape;}, howpublished = {tištěná verze "print"}, language = {eng}, location = {New York}, isbn = {978-1-62703-642-9}, pages = {53-66}, publisher = {Humana Press}, title = {Automated Microscopy in Forward Genetic Screening of Arabidopsis}, url = {http://link.springer.com/protocol/10.1007%2F978-1-62703-643-6_4}, year = {2014} }
TY - JOUR ID - 1230832 AU - Dobisová, Tereza - Hejátko, Jan PY - 2014 TI - Automated Microscopy in Forward Genetic Screening of Arabidopsis PB - Humana Press CY - New York SN - 9781627036429 KW - apical meristem KW - Arabidopsis thaliana KW - branching morphogenesis KW - cell communication KW - cell function KW - cell membrane KW - cell shape; UR - http://link.springer.com/protocol/10.1007%2F978-1-62703-643-6_4 L2 - http://link.springer.com/protocol/10.1007%2F978-1-62703-643-6_4 N2 - Tightly controlled spatiotemporal specificity of gene expression is intrinsic to developmental and adaptation responses of living systems throughout the kingdoms. Forward genetic screens employing well-characterized reporter lines can be used to identify as yet unknown genetic factors driving specific aspects of individual regulatory pathways. However, such screens are demanding with respect to data acquisition and analysis from thousands of mutant lines. Here, we describe a method that allows screening of a mutagenized GUS reporter line in Arabidopsis using an automated microscopy imaging system as a tool for rapid and efficient identification of mutants with modified expression profile for a gene of interest. ER -
DOBISOVÁ, Tereza and Jan HEJÁTKO. Automated Microscopy in Forward Genetic Screening of Arabidopsis. In Žárský V. \textit{Plant Cell Morphogenesis: Methods and Protocols}. New York: Humana Press, 2014, p.~53-66. ISBN~978-1-62703-642-9. Available from: https://dx.doi.org/10.1007/978-1-62703-643-6\_{}4.
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