Automated Microscopy in Forward Genetic Screening of
Arabidopsis

D 2014

Automated Microscopy in Forward Genetic Screening of Arabidopsis

DOBISOVÁ, Tereza and Jan HEJÁTKO

Basic information

Original name

Automated Microscopy in Forward Genetic Screening of Arabidopsis

Authors

DOBISOVÁ, Tereza (203 Czech Republic, belonging to the institution) and Jan HEJÁTKO (203 Czech Republic, guarantor, belonging to the institution)

Edition

New York, Plant Cell Morphogenesis: Methods and Protocols, p. 53-66, 14 pp. 2014

Publisher

Humana Press

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

printed version "print"

References:

URL

RIV identification code

RIV/00216224:14740/14:00080711

Organization unit

Central European Institute of Technology

ISBN

978-1-62703-642-9

ISSN

DOI

http://dx.doi.org/10.1007/978-1-62703-643-6_4

UT WoS

000326510400005

Keywords in English

apical meristem; Arabidopsis thaliana; branching morphogenesis; cell communication; cell function; cell membrane; cell shape;

Abstract

V originále

Tightly controlled spatiotemporal specificity of gene expression is intrinsic to developmental and adaptation responses of living systems throughout the kingdoms. Forward genetic screens employing well-characterized reporter lines can be used to identify as yet unknown genetic factors driving specific aspects of individual regulatory pathways. However, such screens are demanding with respect to data acquisition and analysis from thousands of mutant lines. Here, we describe a method that allows screening of a mutagenized GUS reporter line in Arabidopsis using an automated microscopy imaging system as a tool for rapid and efficient identification of mutants with modified expression profile for a gene of interest.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

GAP501/11/1150, research and development project

Name: Význam interakcí cytokininů a auxinu v regulaci gravitropismu kořene u Arabidopsis thaliana.

Investor: Czech Science Foundation

GA13-25280S, research and development project

Name: Elucidating molecular mechanisms of cytokinins-ethylene crosstalk in the plant development

Investor: Czech Science Foundation

Citovat

DOBISOVÁ, Tereza and Jan HEJÁTKO. Automated Microscopy in Forward Genetic Screening of Arabidopsis. In Žárský V. Plant Cell Morphogenesis: Methods and Protocols. New York: Humana Press, 2014, p. 53-66. ISBN 978-1-62703-642-9. Available from: https://dx.doi.org/10.1007/978-1-62703-643-6_4.

@inproceedings{1230832,
   author = {Dobisová, Tereza and Hejátko, Jan},
   address = {New York},
   booktitle = {Plant Cell Morphogenesis: Methods and Protocols},
   doi = {http://dx.doi.org/10.1007/978-1-62703-643-6_4},
   editor = {Žárský V.},
   keywords = {apical meristem; Arabidopsis thaliana; branching morphogenesis; cell communication; cell function; cell membrane; cell shape;},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {New York},
   isbn = {978-1-62703-642-9},
   pages = {53-66},
   publisher = {Humana Press},
   title = {Automated Microscopy in Forward Genetic Screening of Arabidopsis},
   url = {http://link.springer.com/protocol/10.1007%2F978-1-62703-643-6_4},
   year = {2014}
}

TY  - JOUR
ID  - 1230832
AU  - Dobisová, Tereza - Hejátko, Jan
PY  - 2014
TI  - Automated Microscopy in Forward Genetic Screening of Arabidopsis
PB  - Humana Press
CY  - New York
SN  - 9781627036429
KW  - apical meristem
KW  - Arabidopsis thaliana
KW  - branching morphogenesis
KW  - cell communication
KW  - cell function
KW  - cell membrane
KW  - cell shape;
UR  - http://link.springer.com/protocol/10.1007%2F978-1-62703-643-6_4
L2  - http://link.springer.com/protocol/10.1007%2F978-1-62703-643-6_4
N2  - Tightly controlled spatiotemporal specificity of gene expression is intrinsic to developmental and adaptation responses of living systems throughout the kingdoms. Forward genetic screens employing well-characterized reporter lines can be used to identify as yet unknown genetic factors driving specific aspects of individual regulatory pathways. However, such screens are demanding with respect to data acquisition and analysis from thousands of mutant lines. Here, we describe a method that allows screening of a mutagenized GUS reporter line in Arabidopsis using an automated microscopy imaging system as a tool for rapid and efficient identification of mutants with modified expression profile for a gene of interest.
ER  -

DOBISOVÁ, Tereza and Jan HEJÁTKO. Automated Microscopy in Forward Genetic Screening of Arabidopsis. In Žárský V. \textit{Plant Cell Morphogenesis: Methods and Protocols}. New York: Humana Press, 2014, p.~53-66. ISBN~978-1-62703-642-9. Available from: https://dx.doi.org/10.1007/978-1-62703-643-6\_{}4.

Detailed Information on Publication Record