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@inproceedings{1230832,
author = {Dobisová, Tereza and Hejátko, Jan},
address = {New York},
booktitle = {Plant Cell Morphogenesis: Methods and Protocols},
doi = {http://dx.doi.org/10.1007/978-1-62703-643-6_4},
editor = {Žárský V.},
keywords = {apical meristem; Arabidopsis thaliana; branching morphogenesis; cell communication; cell function; cell membrane; cell shape;},
howpublished = {tištěná verze "print"},
language = {eng},
location = {New York},
isbn = {978-1-62703-642-9},
pages = {53-66},
publisher = {Humana Press},
title = {Automated Microscopy in Forward Genetic Screening of Arabidopsis},
url = {http://link.springer.com/protocol/10.1007%2F978-1-62703-643-6_4},
year = {2014}
}
TY - JOUR
ID - 1230832
AU - Dobisová, Tereza - Hejátko, Jan
PY - 2014
TI - Automated Microscopy in Forward Genetic Screening of Arabidopsis
PB - Humana Press
CY - New York
SN - 9781627036429
KW - apical meristem
KW - Arabidopsis thaliana
KW - branching morphogenesis
KW - cell communication
KW - cell function
KW - cell membrane
KW - cell shape;
UR - http://link.springer.com/protocol/10.1007%2F978-1-62703-643-6_4
L2 - http://link.springer.com/protocol/10.1007%2F978-1-62703-643-6_4
N2 - Tightly controlled spatiotemporal specificity of gene expression is intrinsic to developmental and adaptation responses of living systems throughout the kingdoms. Forward genetic screens employing well-characterized reporter lines can be used to identify as yet unknown genetic factors driving specific aspects of individual regulatory pathways. However, such screens are demanding with respect to data acquisition and analysis from thousands of mutant lines. Here, we describe a method that allows screening of a mutagenized GUS reporter line in Arabidopsis using an automated microscopy imaging system as a tool for rapid and efficient identification of mutants with modified expression profile for a gene of interest.
ER -
DOBISOVÁ, Tereza and Jan HEJÁTKO. Automated Microscopy in Forward Genetic Screening of Arabidopsis. In Žárský V. \textit{Plant Cell Morphogenesis: Methods and Protocols}. New York: Humana Press. p.~53-66. ISBN~978-1-62703-642-9. doi:10.1007/978-1-62703-643-6\_{}4. 2014.
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