Detailed Information on Publication Record
2014
Electrophoretic Characterization and Purification of Silica-Coated Photon-Upconverting Nanoparticles and Their Bioconjugates
HLAVÁČEK, Antonín, Andreas SEDLMEIER, Petr SKLÁDAL and Hans Heiner GORRISBasic information
Original name
Electrophoretic Characterization and Purification of Silica-Coated Photon-Upconverting Nanoparticles and Their Bioconjugates
Authors
HLAVÁČEK, Antonín (203 Czech Republic, belonging to the institution), Andreas SEDLMEIER (276 Germany), Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution) and Hans Heiner GORRIS (276 Germany)
Edition
ACS APPLIED MATERIALS & INTERFACES, WASHINGTON, AMER CHEMICAL SOC, 2014, 1944-8244
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 6.723
RIV identification code
RIV/00216224:14740/14:00079627
Organization unit
Central European Institute of Technology
UT WoS
000336075300111
Keywords in English
bioconjugation; electrophoresis; nanoparticles; silica coating; upconversion
Tags
Tags
International impact, Reviewed
Změněno: 2/4/2015 09:52, Martina Prášilová
V originále
Photon-upconverting nanoparticles (UCNPs) have attracted much interest as a new class of luminescent label for the background-free detection in bioanalytical applications. UCNPs and other nanoparticles are commonly coated with a silica shell to improve their dispersibility and chemical stability in aqueous buffer and to incorporate functional groups for subsequent bioconjugation steps. The process of silica coating, however, is difficult to control without suitable analytical and preparative methods. Here, we have introduced agarose gel electrophoresis for the analysis and purification of silica-coated UCNPs. The silica shell can be doped with a fluorescent dye for direct detection in the gel without influencing the structure or electrophoretic mobility of the nanoparticles. The preparation of a bare silica shell by reverse microemulsion resulted in individual nanoparticles but also distinct aggregates that could be separated and isolated from the agarose gel. In contrast, the preparation of an ultrathin carboxylated silica shell yielded non-aggregated UCNPs only that could be directly used for protein conjugation. Agarose gel electrophoresis has also facilitated an efficient separation of protein UCNP conjugates from excess reagents.
In Czech
Metoda separace fluorescenčních nanočástic na bázi up-konverze a jejich konjugátů.
Links
ED1.1.00/02.0068, research and development project |
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EE2.3.30.0009, research and development project |
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