J 2014

Electrophoretic Characterization and Purification of Silica-Coated Photon-Upconverting Nanoparticles and Their Bioconjugates

HLAVÁČEK, Antonín, Andreas SEDLMEIER, Petr SKLÁDAL and Hans Heiner GORRIS

Basic information

Original name

Electrophoretic Characterization and Purification of Silica-Coated Photon-Upconverting Nanoparticles and Their Bioconjugates

Authors

HLAVÁČEK, Antonín (203 Czech Republic, belonging to the institution), Andreas SEDLMEIER (276 Germany), Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution) and Hans Heiner GORRIS (276 Germany)

Edition

ACS APPLIED MATERIALS & INTERFACES, WASHINGTON, AMER CHEMICAL SOC, 2014, 1944-8244

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 6.723

RIV identification code

RIV/00216224:14740/14:00079627

Organization unit

Central European Institute of Technology

UT WoS

000336075300111

Keywords in English

bioconjugation; electrophoresis; nanoparticles; silica coating; upconversion

Tags

International impact, Reviewed
Změněno: 2/4/2015 09:52, Martina Prášilová

Abstract

V originále

Photon-upconverting nanoparticles (UCNPs) have attracted much interest as a new class of luminescent label for the background-free detection in bioanalytical applications. UCNPs and other nanoparticles are commonly coated with a silica shell to improve their dispersibility and chemical stability in aqueous buffer and to incorporate functional groups for subsequent bioconjugation steps. The process of silica coating, however, is difficult to control without suitable analytical and preparative methods. Here, we have introduced agarose gel electrophoresis for the analysis and purification of silica-coated UCNPs. The silica shell can be doped with a fluorescent dye for direct detection in the gel without influencing the structure or electrophoretic mobility of the nanoparticles. The preparation of a bare silica shell by reverse microemulsion resulted in individual nanoparticles but also distinct aggregates that could be separated and isolated from the agarose gel. In contrast, the preparation of an ultrathin carboxylated silica shell yielded non-aggregated UCNPs only that could be directly used for protein conjugation. Agarose gel electrophoresis has also facilitated an efficient separation of protein UCNP conjugates from excess reagents.

In Czech

Metoda separace fluorescenčních nanočástic na bázi up-konverze a jejich konjugátů.

Links

ED1.1.00/02.0068, research and development project
Name: CEITEC - central european institute of technology
EE2.3.30.0009, research and development project
Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci