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@article{1231045, author = {Šebrlová, Kristýna and Peš, Ondřej and Slaninová, Iva and Vymazal, Ondřej and Kantorová, Jana and Táborská, Eva}, article_location = {Berlin}, article_number = {5}, doi = {http://dx.doi.org/10.1515/chempap-2015-0083}, keywords = {Stylophorum lasiocarpum; traditional Chinese medicine; vegetation period; benzophenanthridine; LC MS; cytotoxicity}, language = {eng}, issn = {0366-6352}, journal = {Chemical papers}, title = {Seasonal variation in alkaloid composition and antiproliferative activity of Stylophorum lasiocarpum (Oliv.) Fedde}, url = {http://www.chemicalpapers.com/?id=7&paper=8475}, volume = {69}, year = {2015} }
TY - JOUR ID - 1231045 AU - Šebrlová, Kristýna - Peš, Ondřej - Slaninová, Iva - Vymazal, Ondřej - Kantorová, Jana - Táborská, Eva PY - 2015 TI - Seasonal variation in alkaloid composition and antiproliferative activity of Stylophorum lasiocarpum (Oliv.) Fedde JF - Chemical papers VL - 69 IS - 5 SP - 698-708 EP - 698-708 PB - WALTER DE GRUYTER GMBH SN - 03666352 KW - Stylophorum lasiocarpum KW - traditional Chinese medicine KW - vegetation period KW - benzophenanthridine KW - LC MS KW - cytotoxicity UR - http://www.chemicalpapers.com/?id=7&paper=8475 N2 - Stylophorum lasiocarpum (Oliv.) Fedde (Papaveraceae) belongs to traditional Chinese medicine herbs but there was minimal information on the content of alkaloids in this plant. Extracts from the aerial part and roots were examined by liquid chromatography with UV and mass spectrometric detection, with nineteen alkaloids identified. Changes in alkaloid content over the entire vegetation period of a one- and two-year old plant were studied. The protoberberine alkaloids, coptisine and stylopine, were found to be the main substances in extracts of the aerial part irrespective of the plant’s age and time of harvest. Variable amounts of protopine, sanguinarine, chelerythrine, chelirubine, macarpine, chelilutine and berberine were also recorded in the aerial part. The roots contained significantly larger quantities of all alkaloids than the aerial part with the levels of most alkaloids varying from May to October, peaking in the middle of the vegetation period. Coptisine was the dominant alkaloid in all samples. The antiproliferative activities of the root extract and of seven individual alkaloids were tested on A375 human malignant melanoma cells. The significant dose-dependent toxicity of the root extract was attributed largely to the quaternary benzo[c]phenanthridine alkaloids, macarpine and sanguinarine. ER -
ŠEBRLOVÁ, Kristýna, Ondřej PEŠ, Iva SLANINOVÁ, Ondřej VYMAZAL, Jana KANTOROVÁ and Eva TÁBORSKÁ. Seasonal variation in alkaloid composition and antiproliferative activity of Stylophorum lasiocarpum (Oliv.) Fedde. \textit{Chemical papers}. Berlin: WALTER DE GRUYTER GMBH, 2015, vol.~69, No~5, p.~698-708. ISSN~0366-6352. Available from: https://dx.doi.org/10.1515/chempap-2015-0083.
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