Další formáty:
BibTeX
LaTeX
RIS
@article{1232507, author = {Manakhov, Anton and Skládal, Petr and Nečas, David and Čechal, Jan and Polčák, Josef and Eliáš, Marek and Zajíčková, Lenka}, article_location = {WEINHEIM}, article_number = {12}, doi = {http://dx.doi.org/10.1002/pssa.201431399}, keywords = {amine plasma; biosensors; polymers; quartz crystal microbalance; X-ray photoelectron spectroscopy}, language = {eng}, issn = {1862-6300}, journal = {PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE}, title = {Cyclopropylamine plasma polymers deposited onto quartz crystal microbalance for biosensing application}, url = {http://onlinelibrary.wiley.com/doi/10.1002/pssa.201431399/epdf}, volume = {211}, year = {2014} }
TY - JOUR ID - 1232507 AU - Manakhov, Anton - Skládal, Petr - Nečas, David - Čechal, Jan - Polčák, Josef - Eliáš, Marek - Zajíčková, Lenka PY - 2014 TI - Cyclopropylamine plasma polymers deposited onto quartz crystal microbalance for biosensing application JF - PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE VL - 211 IS - 12 SP - 2801-2808 EP - 2801-2808 PB - WILEY-V C H VERLAG GMBH SN - 18626300 KW - amine plasma KW - biosensors KW - polymers KW - quartz crystal microbalance KW - X-ray photoelectron spectroscopy UR - http://onlinelibrary.wiley.com/doi/10.1002/pssa.201431399/epdf L2 - http://onlinelibrary.wiley.com/doi/10.1002/pssa.201431399/epdf N2 - Biosensors have been extensively developed and applied for biomedical and environmental studies. Although there are many different types of biosensing techniques, all these methods require immobilization of biomolecules. Therefore, this is a critical issue in the development of any sort of biosensor affecting its operational properties. In this work, we address this critical issue by employing cyclopropylamine plasma polymerization to deposit stable amine thin films on the surface of quartz crystal microbalance (QCM) biosensors. The antibody specific to human serum albumin (anti-HSA) is attached to the QCM surface via cross-linkage obtained by intermediate reaction with glutaraldehyde. All steps of the bio-immobilization are controlled by XPS and FTIR to characterize surface and layer (bulk) chemistry. The results obtained with the antibody linked through the CPA plasma-polymerized layer are comparable with the results obtained previously when the same antibody AL-01 was linked to the surface using the self-assembled thiol monolayer based on cystamine. [GRAPHICS] (C) 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim ER -
MANAKHOV, Anton, Petr SKLÁDAL, David NEČAS, Jan ČECHAL, Josef POLČÁK, Marek ELIÁŠ a Lenka ZAJÍČKOVÁ. Cyclopropylamine plasma polymers deposited onto quartz crystal microbalance for biosensing application. \textit{PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE}. WEINHEIM: WILEY-V C H VERLAG GMBH, roč.~211, č.~12, s.~2801-2808. ISSN~1862-6300. doi:10.1002/pssa.201431399. 2014.
|