MANAKHOV, Anton, Petr SKLÁDAL, David NEČAS, Jan ČECHAL, Josef POLČÁK, Marek ELIÁŠ and Lenka ZAJÍČKOVÁ. Cyclopropylamine plasma polymers deposited onto quartz crystal microbalance for biosensing application. PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE. WEINHEIM: WILEY-V C H VERLAG GMBH, 2014, vol. 211, No 12, p. 2801-2808. ISSN 1862-6300. doi:10.1002/pssa.201431399.
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Basic information
Original name Cyclopropylamine plasma polymers deposited onto quartz crystal microbalance for biosensing application
Authors MANAKHOV, Anton (643 Russian Federation, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution), David NEČAS (203 Czech Republic, belonging to the institution), Jan ČECHAL (203 Czech Republic), Josef POLČÁK (203 Czech Republic), Marek ELIÁŠ (203 Czech Republic, belonging to the institution) and Lenka ZAJÍČKOVÁ (203 Czech Republic, guarantor, belonging to the institution).
Edition PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE, WEINHEIM, WILEY-V C H VERLAG GMBH, 2014, 1862-6300.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10302 Condensed matter physics
Country of publisher Germany
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 1.616
RIV identification code RIV/00216224:14740/14:00079904
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1002/pssa.201431399
UT WoS 000347182700021
Keywords in English amine plasma; biosensors; polymers; quartz crystal microbalance; X-ray photoelectron spectroscopy
Tags kontrola MP, MP, podil, rivok
Tags International impact, Reviewed
Changed by Changed by: Martina Prášilová, učo 342282. Changed: 30. 3. 2015 11:43.
Abstract
Biosensors have been extensively developed and applied for biomedical and environmental studies. Although there are many different types of biosensing techniques, all these methods require immobilization of biomolecules. Therefore, this is a critical issue in the development of any sort of biosensor affecting its operational properties. In this work, we address this critical issue by employing cyclopropylamine plasma polymerization to deposit stable amine thin films on the surface of quartz crystal microbalance (QCM) biosensors. The antibody specific to human serum albumin (anti-HSA) is attached to the QCM surface via cross-linkage obtained by intermediate reaction with glutaraldehyde. All steps of the bio-immobilization are controlled by XPS and FTIR to characterize surface and layer (bulk) chemistry. The results obtained with the antibody linked through the CPA plasma-polymerized layer are comparable with the results obtained previously when the same antibody AL-01 was linked to the surface using the self-assembled thiol monolayer based on cystamine. [GRAPHICS] (C) 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
286154, interní kód MUName: SYLICA - Synergies of Life and Material Sciences to Create a New Future (Acronym: SYLICA)
Investor: European Union, Capacities
3SGA5652, interní kód MUName: Bioactive Nanofibers Prepared by Electrospinning and Plasma Technologies (Acronym: BioFibPlas)
Investor: South-Moravian Region, Incoming grants
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