Labour mobility (or, formally, travels to work) is the most significant region-shaping process creating agglomeration hinterlands of all larger cities. The South-Moravian Region and the city of Brno, which, with the population of 400,000, is its natural centre, represent the model region for this article. Based on performed research work the article would like to introduce and verify the potential of labour mobility of the region's inhabitants in relation to the expected potential salary/wage, transport time and distance from the centre - these belong among the principal economic motivators for travelling to work. Agglomeration effects of the Brno City are therefore perceived in the context of space and time while emphasizing transport costs, transport facilities and the effect of labour mobility itself.