Inflammatory profiling of Schwann cells in contact with growing axons distal to nerve injury

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ and Ivana HRADILOVÁ SVÍŽENSKÁ. Inflammatory profiling of Schwann cells in contact with growing axons distal to nerve injury. In International Symposium on Peripheral Nerve Regeneration, Torino. 2014.

Basic information

• Original name: Inflammatory profiling of Schwann cells in contact with growing axons distal to nerve injury
• Name in Czech: Zánětlivá profilace Schwanových buněk v kontaktu s rostoucími axony distálně od poškození periferního nervu
• Authors: DUBOVÝ, Petr, Ilona KLUSÁKOVÁ and Ivana HRADILOVÁ SVÍŽENSKÁ.
• Edition: International Symposium on Peripheral Nerve Regeneration, Torino, 2014.

Other information

• Original language: English
• Type of outcome: Requested lectures
• Field of Study: 30000 3. Medical and Health Sciences
• Country of publisher: Italy
• Confidentiality degree: is not subject to a state or trade secret
• Keywords (in Czech): peripheral neuropatic pain model, Wallerian degeneration, activated Schwann cells, cytokines, macrophages
• Keywords in English: model periferní neuropatické bolesti, Wallerova degenerace, aktivované Schwannovy buňky, cytokiny, makrofágy

Abstract

Wallerian degeneration is a cascade of stereotype cellular and molecular events distal to injury of nerve fibers considered to be a sort of innate immune reaction or neuroinflammation. The cellular events comprise inflammatory activation of Schwann cells and invasion of various types of immune cells including macrophages that produce cytokines and chemokines. However, little is known on beneficial effect of proinflammatory cytokines upregulated by activated Schwann cells. Therefore, the goal of present study was to investigate expression of proinflammatory cytokines (IL-1b, TNFa, IL-6) and their receptors in activated Schwann cells distal to crush and compression injury of rat sciatic nerves.The sciatic nerve sections from naïve or sham-operated rats displayed very week immunostaining for investigated cytokines and their receptors. Immunofluorescence of significantly higher intensity was found in sections distal to nerve lesion as well as in sections of contralateral sciatic nerve. Immunostaining revealed that proinflammatory cytokines and their receptors are dominantly expressed by activated Schwann cells which many of them were in close contact with growing axons. Surprisingly, activated ED-1+ macrophages in distal sciatic nerve segments displayed significantly lower level of proinflammatory cytokines and their receptors.

Links

• CZ.1.05/1.1.00/02.0068, interní kód MU: Name: CEITEC - středoevropský technologický institut (Acronym: CEITEC)

Investor: Ministry of Education, Youth and Sports of the CR, CEITEC - Central European Institute of Technology, 1.1 European Centres of Excellence

• CZ.1.07/2.4.00/17.0042, interní kód MU: Name: InterBioNet - Podpora mezinárodní a mezisektorové spolupráce ve výzkumu a vývoji v oblasti věd o živé přírodě

Investor: Ministry of Education, Youth and Sports of the CR, 2.4 Partnership and networks