| KOMINKOVA, Marketa, Petr MICHALEK, Kristyna CIHALOVA, Roman GURAN, Natalia Vladimirovna CERNEI, Lukáš NEJDL, Kristyna SMERKOVA, Simona DOSTALOVA, Dagmar CHUDOBOVA, Zbynek HEGER, Radek VESELÝ, Jaromír GUMULEC, Jindrich KYNICKY, Kledi XHAXHIU, Ondrej ZITKA, Vojtěch ADAM a Rene KIZEK. Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients' Swabs. International Journal of Molecular Sciences. Basel: MDPI AG, 2015, roč. 16, č. 4, s. 7210-7229. ISSN 1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms16047210. |
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@article{1232672,
author = {Kominkova, Marketa and Michalek, Petr and Cihalova, Kristyna and Guran, Roman and Cernei, Natalia Vladimirovna and Nejdl, Lukáš and Smerkova, Kristyna and Dostalova, Simona and Chudobova, Dagmar and Heger, Zbynek and Veselý, Radek and Gumulec, Jaromír and Kynicky, Jindrich and Xhaxhiu, Kledi and Zitka, Ondrej and Adam, Vojtěch and Kizek, Rene},
article_location = {Basel},
article_number = {4},
doi = {http://dx.doi.org/10.3390/ijms16047210},
keywords = {buthionine sulfoximine; Escherichia coli; glutathione; infections; swabs},
language = {eng},
issn = {1422-0067},
journal = {International Journal of Molecular Sciences},
title = {Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients' Swabs},
url = {http://www.mdpi.com/1422-0067/16/4/7210},
volume = {16},
year = {2015}
}
TY - JOUR
ID - 1232672
AU - Kominkova, Marketa - Michalek, Petr - Cihalova, Kristyna - Guran, Roman - Cernei, Natalia Vladimirovna - Nejdl, Lukáš - Smerkova, Kristyna - Dostalova, Simona - Chudobova, Dagmar - Heger, Zbynek - Veselý, Radek - Gumulec, Jaromír - Kynicky, Jindrich - Xhaxhiu, Kledi - Zitka, Ondrej - Adam, Vojtěch - Kizek, Rene
PY - 2015
TI - Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients' Swabs
JF - International Journal of Molecular Sciences
VL - 16
IS - 4
SP - 7210-7229
EP - 7210-7229
PB - MDPI AG
SN - 14220067
KW - buthionine sulfoximine
KW - Escherichia coli
KW - glutathione
KW - infections
KW - swabs
UR - http://www.mdpi.com/1422-0067/16/4/7210
N2 - In this work, we focused on the differences between bacterial cultures of E. coli obtained from swabs of infectious wounds of patients compared to laboratory E. coli. In addition, blocking of the protein responsible for the synthesis of glutathione (gamma-glutamylcysteine synthase—GCL) using 10 mM buthionine sulfoximine was investigated. Each E. coli showed significant differences in resistance to antibiotics. According to the determined resistance, E. coli were divided into experimental groups based on a statistical evaluation of their properties as more resistant and more sensitive. These groups were also used for finding the differences in a dependence of the glutathione pathway on resistance to antibiotics. More sensitive E. coli showed the same kinetics of glutathione synthesis while blocking GCL (Km 0.1 µM), as compared to non-blocking. In addition, the most frequent mutations in genes of glutathione synthetase, glutathione peroxidase and glutathione reductase were observed in this group compared to laboratory E.coli. The group of “more resistant” E. coli exhibited differences in Km between 0.3 and 0.8 µM. The number of mutations compared to the laboratory E. coli was substantially lower compared to the other group.
ER -
KOMINKOVA, Marketa, Petr MICHALEK, Kristyna CIHALOVA, Roman GURAN, Natalia Vladimirovna CERNEI, Lukáš NEJDL, Kristyna SMERKOVA, Simona DOSTALOVA, Dagmar CHUDOBOVA, Zbynek HEGER, Radek VESELÝ, Jaromír GUMULEC, Jindrich KYNICKY, Kledi XHAXHIU, Ondrej ZITKA, Vojtěch ADAM a Rene KIZEK. Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients' Swabs. \textit{International Journal of Molecular Sciences}. Basel: MDPI AG, 2015, roč.~16, č.~4, s.~7210-7229. ISSN~1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms16047210.
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