Localized movement and morphology of UBF1-positive nucleolar
regions are changed by gamma-irradiation in G2 phase of the
cell cycle

J 2015

Localized movement and morphology of UBF1-positive nucleolar regions are changed by gamma-irradiation in G2 phase of the cell cycle

SOROKIN, Dmitry, Lenka STIXOVÁ, Petra SEHNALOVÁ, Soňa LEGARTOVÁ, Jana SUCHÁNKOVÁ et. al.

Basic information

Original name

Localized movement and morphology of UBF1-positive nucleolar regions are changed by gamma-irradiation in G2 phase of the cell cycle

Authors

SOROKIN, Dmitry (643 Russian Federation, guarantor, belonging to the institution), Lenka STIXOVÁ (203 Czech Republic), Petra SEHNALOVÁ (203 Czech Republic), Soňa LEGARTOVÁ (703 Slovakia), Jana SUCHÁNKOVÁ (203 Czech Republic), Pavel ŠIMARA (203 Czech Republic, belonging to the institution), Stanislav KOZUBEK (203 Czech Republic), Pavel MATULA (203 Czech Republic, belonging to the institution), Magdalena SKALNÍKOVÁ (203 Czech Republic), Ivan RAŠKA (203 Czech Republic) and Eva BÁRTOVÁ (203 Czech Republic)

Edition

Nucleus, 2015, 1949-1034

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.446

RIV identification code

RIV/00216224:14330/15:00080738

Organization unit

Faculty of Informatics

DOI

http://dx.doi.org/10.1080/19491034.2015.1075111

UT WoS

000360666000008

Keywords in English

DNA damage; live cells; nucleolus; uncleoli tracking; UBF1

Abstract

V originále

The nucleolus is a well-organized site of ribosomal gene transcription. Moreover, many DNA repair pathway proteins, including ATM, ATR kinases, MRE11, PARP1 and Ku70/80, localize to the nucleolus (Moore et al., 2011). We analyzed the consequences of DNA damage in nucleoli following ultraviolet A (UVA), C (UVC), or g-irradiation in order to test whether and how radiation-mediated genome injury affects local motion and morphology of nucleoli. Because exposure to radiation sources can induce changes in the pattern of UBF1-positive nucleolar regions, we visualized nucleoli in living cells by GFP-UBF1 expression for subsequent morphological analyses and local motion studies. UVA radiation, but not 5 Gy of g-rays, induced apoptosis as analyzed by an advanced computational method. In non-apoptotic cells, we observed that gradiation caused nucleolar re-positioning over time and changed several morphological parameters, including the size of the nucleolus and the area of individual UBF1-positive foci. Radiation-induced nucleoli rearrangement was observed particularly in G2 phase of the cell cycle, indicating repair of ribosomal genes in G2 phase and implying that nucleoli are less stable, thus sensitive to radiation, in G2 phase.

Links

CZ.1.07/2.3.00/30.0030, interní kód MU

Name: Rozvoj lidských zdrojů pro oblast buněčné biologie

Investor: Ministry of Education, Youth and Sports of the CR, 2.3 Human resources in research and development

GBP302/12/G157, research and development project

Name: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Czech Science Foundation

Citovat

SOROKIN, Dmitry, Lenka STIXOVÁ, Petra SEHNALOVÁ, Soňa LEGARTOVÁ, Jana SUCHÁNKOVÁ, Pavel ŠIMARA, Stanislav KOZUBEK, Pavel MATULA, Magdalena SKALNÍKOVÁ, Ivan RAŠKA and Eva BÁRTOVÁ. Localized movement and morphology of UBF1-positive nucleolar regions are changed by gamma-irradiation in G2 phase of the cell cycle. Nucleus. 2015, vol. 6, No 4, p. 301-313. ISSN 1949-1034. Available from: https://dx.doi.org/10.1080/19491034.2015.1075111.

@article{1232675,
   author = {Sorokin, Dmitry and Stixová, Lenka and Sehnalová, Petra and Legartová, Soňa and Suchánková, Jana and Šimara, Pavel and Kozubek, Stanislav and Matula, Pavel and Skalníková, Magdalena and Raška, Ivan and Bártová, Eva},
   article_number = {4},
   doi = {http://dx.doi.org/10.1080/19491034.2015.1075111},
   keywords = {DNA damage; live cells; nucleolus; uncleoli tracking; UBF1},
   language = {eng},
   issn = {1949-1034},
   journal = {Nucleus},
   title = {Localized movement and morphology of UBF1-positive nucleolar regions are changed by gamma-irradiation in G2 phase of the cell cycle},
   volume = {6},
   year = {2015}
}

TY  - JOUR
ID  - 1232675
AU  - Sorokin, Dmitry - Stixová, Lenka - Sehnalová, Petra - Legartová, Soňa - Suchánková, Jana - Šimara, Pavel - Kozubek, Stanislav - Matula, Pavel - Skalníková, Magdalena - Raška, Ivan - Bártová, Eva
PY  - 2015
TI  - Localized movement and morphology of UBF1-positive nucleolar regions are changed by gamma-irradiation in G2 phase of the cell cycle
JF  - Nucleus
VL  - 6
IS  - 4
SP  - 301-313
EP  - 301-313
SN  - 19491034
KW  - DNA damage
KW  - live cells
KW  - nucleolus
KW  - uncleoli tracking
KW  - UBF1
N2  - The nucleolus is a well-organized site of ribosomal gene transcription. Moreover, many DNA repair pathway proteins, including ATM, ATR kinases, MRE11, PARP1 and Ku70/80, localize to the nucleolus (Moore et al., 2011). We analyzed the consequences of DNA damage in nucleoli following ultraviolet A (UVA), C (UVC), or g-irradiation in order to test whether and how radiation-mediated genome injury affects local motion and morphology of nucleoli. Because exposure to radiation sources can induce changes in the pattern of UBF1-positive nucleolar regions, we visualized nucleoli in living cells by GFP-UBF1 expression for subsequent morphological analyses and local motion studies. UVA radiation, but not 5 Gy of g-rays, induced apoptosis as analyzed by an advanced computational method. In non-apoptotic cells, we observed that gradiation caused nucleolar re-positioning over time and changed several morphological parameters, including the size of the nucleolus and the area of individual UBF1-positive foci. Radiation-induced nucleoli rearrangement was observed particularly in G2 phase of the cell cycle, indicating repair of ribosomal genes in G2 phase and implying that nucleoli are less stable, thus sensitive to radiation, in G2 phase.
ER  -

SOROKIN, Dmitry, Lenka STIXOVÁ, Petra SEHNALOVÁ, Soňa LEGARTOVÁ, Jana SUCHÁNKOVÁ, Pavel ŠIMARA, Stanislav KOZUBEK, Pavel MATULA, Magdalena SKALNÍKOVÁ, Ivan RAŠKA and Eva BÁRTOVÁ. Localized movement and morphology of UBF1-positive nucleolar regions are changed by gamma-irradiation in G2 phase of the cell cycle. \textit{Nucleus}. 2015, vol.~6, No~4, p.~301-313. ISSN~1949-1034. Available from: https://dx.doi.org/10.1080/19491034.2015.1075111.

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