RNA-dependent disassembly of nuclear bodies

J 2016

RNA-dependent disassembly of nuclear bodies

MUSINOVA, Yana R., Olga M. LISITSYNA, Dmitry SOROKIN, Eugene A. ARIFULIN, Tatiana A. SMIRNOVA et. al.

Basic information

Original name

RNA-dependent disassembly of nuclear bodies

Authors

MUSINOVA, Yana R. (643 Russian Federation), Olga M. LISITSYNA (643 Russian Federation), Dmitry SOROKIN (643 Russian Federation, guarantor, belonging to the institution), Eugene A. ARIFULIN (643 Russian Federation), Tatiana A. SMIRNOVA (643 Russian Federation), Roman A. ZINOVKIN (643 Russian Federation), Daria M. POTASHNIKOVA (643 Russian Federation), Yegor S. VASSETZKY (643 Russian Federation) and Eugene V. SHEVAL (643 Russian Federation)

Edition

Journal of Cell Science, The Company of Biologists Ltd. 2016, 0021-9533

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 4.431

RIV identification code

RIV/00216224:14330/16:00087720

Organization unit

Faculty of Informatics

DOI

http://dx.doi.org/10.1242/jcs.189142

UT WoS

000391103700008

Keywords in English

Nuclear bodies; Nucleolus; Interphase prenucleolar body; pre-rRNA; B23; NPM1; Self-organization

Abstract

V originále

Nuclear bodies are membraneless organelles that play important roles in genome functioning. A specific type of nuclear bodies known as interphase prenucleolar bodies (iPNBs) are formed in the nucleoplasm after hypotonic stress from partially disassembled nucleoli. iPNBs are then disassembled, and the nucleoli are reformed simultaneously. Here, we show that diffusion of B23 molecules (also known as nucleophosmin, NPM1) from iPNBs, but not fusion of iPNBs with the nucleoli, contributes to the transfer of B23 from iPNBs to the nucleoli. Maturation of pre-ribosomal RNAs (rRNAs) and the subsequent outflow of mature rRNAs from iPNBs led to the disassembly of iPNBs. We found that B23 transfer was dependent on the synthesis of pre-rRNA molecules in nucleoli; these pre-rRNA molecules interacted with B23 and led to its accumulation within nucleoli. The transfer of B23 between iPNBs and nucleoli was accomplished through a nucleoplasmic pool of B23, and increased nucleoplasmic B23 content retarded disassembly, whereas B23 depletion accelerated disassembly. Our results suggest that iPNB disassembly and nucleolus assembly might be coupled through RNA-dependent exchange of nucleolar proteins, creating a highly dynamic system with long-distance correlations between spatially distinct processes.

Links

GBP302/12/G157, research and development project

Name: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Czech Science Foundation

Citovat

MUSINOVA, Yana R., Olga M. LISITSYNA, Dmitry SOROKIN, Eugene A. ARIFULIN, Tatiana A. SMIRNOVA, Roman A. ZINOVKIN, Daria M. POTASHNIKOVA, Yegor S. VASSETZKY and Eugene V. SHEVAL. RNA-dependent disassembly of nuclear bodies. Journal of Cell Science. The Company of Biologists Ltd., 2016, vol. 129, No 24, p. 4509-4520. ISSN 0021-9533. Available from: https://dx.doi.org/10.1242/jcs.189142.

@article{1232676,
   author = {Musinova, Yana R. and Lisitsyna, Olga M. and Sorokin, Dmitry and Arifulin, Eugene A. and Smirnova, Tatiana A. and Zinovkin, Roman A. and Potashnikova, Daria M. and Vassetzky, Yegor S. and Sheval, Eugene V.},
   article_number = {24},
   doi = {http://dx.doi.org/10.1242/jcs.189142},
   keywords = {Nuclear bodies; Nucleolus; Interphase prenucleolar body; pre-rRNA; B23; NPM1; Self-organization},
   language = {eng},
   issn = {0021-9533},
   journal = {Journal of Cell Science},
   title = {RNA-dependent disassembly of nuclear bodies},
   volume = {129},
   year = {2016}
}

TY  - JOUR
ID  - 1232676
AU  - Musinova, Yana R. - Lisitsyna, Olga M. - Sorokin, Dmitry - Arifulin, Eugene A. - Smirnova, Tatiana A. - Zinovkin, Roman A. - Potashnikova, Daria M. - Vassetzky, Yegor S. - Sheval, Eugene V.
PY  - 2016
TI  - RNA-dependent disassembly of nuclear bodies
JF  - Journal of Cell Science
VL  - 129
IS  - 24
SP  - 4509-4520
EP  - 4509-4520
PB  - The Company of Biologists Ltd.
SN  - 00219533
KW  - Nuclear bodies
KW  - Nucleolus
KW  - Interphase prenucleolar body
KW  - pre-rRNA
KW  - B23
KW  - NPM1
KW  - Self-organization
N2  - Nuclear bodies are membraneless organelles that play important roles in genome functioning. A specific type of nuclear bodies known as interphase prenucleolar bodies (iPNBs) are formed in the nucleoplasm after hypotonic stress from partially disassembled nucleoli. iPNBs are then disassembled, and the nucleoli are reformed simultaneously. Here, we show that diffusion of B23 molecules (also known as nucleophosmin, NPM1) from iPNBs, but not fusion of iPNBs with the nucleoli, contributes to the transfer of B23 from iPNBs to the nucleoli. Maturation of pre-ribosomal RNAs (rRNAs) and the subsequent outflow of mature rRNAs from iPNBs led to the disassembly of iPNBs. We found that B23 transfer was dependent on the synthesis of pre-rRNA molecules in nucleoli; these pre-rRNA molecules interacted with B23 and led to its accumulation within nucleoli. The transfer of B23 between iPNBs and nucleoli was accomplished through a nucleoplasmic pool of B23, and increased nucleoplasmic B23 content retarded disassembly, whereas B23 depletion accelerated disassembly. Our results suggest that iPNB disassembly and nucleolus assembly might be coupled through RNA-dependent exchange of nucleolar proteins, creating a highly dynamic system with long-distance correlations between spatially distinct processes.
ER  -

MUSINOVA, Yana R., Olga M. LISITSYNA, Dmitry SOROKIN, Eugene A. ARIFULIN, Tatiana A. SMIRNOVA, Roman A. ZINOVKIN, Daria M. POTASHNIKOVA, Yegor S. VASSETZKY and Eugene V. SHEVAL. RNA-dependent disassembly of nuclear bodies. \textit{Journal of Cell Science}. The Company of Biologists Ltd., 2016, vol.~129, No~24, p.~4509-4520. ISSN~0021-9533. Available from: https://dx.doi.org/10.1242/jcs.189142.

Detailed Information on Publication Record