Pluripotent stem cell lines display variable hematopoietic
developmental potential

a 2015

Pluripotent stem cell lines display variable hematopoietic developmental potential

TESAŘOVÁ, Lenka, Pavel ŠIMARA, Stanislav STEJSKAL and Irena KRONTORÁD KOUTNÁ

Basic information

Original name

Pluripotent stem cell lines display variable hematopoietic developmental potential

Authors

TESAŘOVÁ, Lenka, Pavel ŠIMARA, Stanislav STEJSKAL and Irena KRONTORÁD KOUTNÁ

Edition

2015 EMBL Symposium: Frontiers in Stem Cells & Cancer, Heidelberg, Germany, Mar 29-31, 2015. 2015

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10601 Cell biology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Informatics

Keywords (in Czech)

pluripotentní kmenové buňky; hematopoetický vývoj

Keywords in English

pluripotent stem cells; hematopoietic development

Abstract

V originále

The clinical application of hematopoietic precursors generated from human pluripotent stem cells (hPSCs) is still limited by low efficient differentiation protocols and functional defects in the derived cells. Moreover, the potential of various hPSCs to differentiate into blood cells has not been examined properly. In this study, protocols for hematopoietic differentiation were applied to available human embryonic stem cell (hESC) and human induced pluripotent stem cell (hiPSC) lines to determine their hematopoietic developmental potential. These protocols included different methods for hPSC cultivation and embryoid bodies (EBs) formation and composition of differentiation media. The efficiency of hematopoietic differentiation was found to be variable among hPSC lines. No protocol optimization enabled generation of CD34+ hematopoietic precursors from available hESC lines CCTL-12 and CCTL-14. On the contrary, these precursors were detected during hiPSC differentiation in basic media supplemented with three cytokines. The yield of precursors was variable, ranging from 0.8 to 10.3 percent of CD34+ cells and from 1.0 to 10,6 percent of CD43+ cells at day seven of differentiation. This yield was further increased when hiPSCs were differentiated for ten days in media with richer cytokine supplement. Nevertheless the variability among hiPSC lines was retained, 6.4 to 16.3 percent of cells were CD34+ and 7.8 to 20.0 percent of cells were CD43+235+ precursors of primitive hematopoiesis, while CD45+ precursors of definitive hematopoiesis comprised only from 0.4 to 4.7 percent. These results indicate that hematopoietic developmental potential of individual hPSC lines is characterised by significant variability that has to be overcome before blood cells derived from hPSC could be used for clinical applications.

Links

CZ.1.07/2.3.00/30.0030, interní kód MU

Name: Rozvoj lidských zdrojů pro oblast buněčné biologie

Investor: Ministry of Education, Youth and Sports of the CR, 2.3 Human resources in research and development

GBP302/12/G157, research and development project

Name: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Czech Science Foundation

Citovat

TESAŘOVÁ, Lenka, Pavel ŠIMARA, Stanislav STEJSKAL and Irena KRONTORÁD KOUTNÁ. Pluripotent stem cell lines display variable hematopoietic developmental potential. In 2015 EMBL Symposium: Frontiers in Stem Cells & Cancer, Heidelberg, Germany, Mar 29-31, 2015. 2015.

@proceedings{1297167,
   author = {Tesařová, Lenka and Šimara, Pavel and Stejskal, Stanislav and Krontorád Koutná, Irena},
   booktitle = {2015 EMBL Symposium: Frontiers in Stem Cells & Cancer, Heidelberg, Germany, Mar 29-31, 2015.},
   keywords = {pluripotent stem cells; hematopoietic development},
   language = {eng},
   title = {Pluripotent stem cell lines display variable hematopoietic developmental potential},
   year = {2015}
}

TY  - CONF
ID  - 1297167
AU  - Tesařová, Lenka - Šimara, Pavel - Stejskal, Stanislav - Krontorád Koutná, Irena
PY  - 2015
TI  - Pluripotent stem cell lines display variable hematopoietic developmental potential
KW  - pluripotent stem cells
KW  - hematopoietic development
N2  - The clinical application of hematopoietic precursors generated from human pluripotent stem cells (hPSCs) is still limited by low efficient differentiation protocols and functional defects in the derived cells. Moreover, the potential of various hPSCs to differentiate into blood cells has not been examined properly. In this study, protocols for hematopoietic differentiation were applied to available human embryonic stem cell (hESC) and human induced pluripotent stem cell (hiPSC) lines to determine their hematopoietic developmental potential. These protocols included different methods for hPSC cultivation and embryoid bodies (EBs) formation and composition of differentiation media. The efficiency of hematopoietic differentiation was found to be variable among hPSC lines. No protocol optimization enabled generation of CD34+ hematopoietic precursors from available hESC lines CCTL-12 and CCTL-14. On the contrary, these precursors were detected during hiPSC differentiation in basic media supplemented with three cytokines. The yield of precursors was variable, ranging from 0.8 to 10.3 percent of CD34+ cells and from 1.0 to 10,6 percent of CD43+ cells at day seven of differentiation. This yield was further increased when hiPSCs were differentiated for ten days in media with richer cytokine supplement. Nevertheless the variability among hiPSC lines was retained, 6.4 to 16.3 percent of cells were CD34+ and 7.8 to 20.0 percent of cells were CD43+235+ precursors of primitive hematopoiesis, while CD45+ precursors of definitive hematopoiesis comprised only from 0.4 to 4.7 percent. These results indicate that hematopoietic developmental potential of individual hPSC lines is characterised by significant variability that has to be overcome before blood cells derived from hPSC could be used for clinical applications.
ER  -

TESAŘOVÁ, Lenka, Pavel ŠIMARA, Stanislav STEJSKAL and Irena KRONTORÁD KOUTNÁ. Pluripotent stem cell lines display variable hematopoietic developmental potential. In \textit{2015 EMBL Symposium: Frontiers in Stem Cells \&{} Cancer, Heidelberg, Germany, Mar 29-31, 2015.}. 2015.

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