a 2015

Single-cell analysis of long-range interactions of POU5F1 foci in hESC cells

STEJSKAL, Stanislav, Bára SZAROWSKÁ, Lenka TESAŘOVÁ, Pavel ŠIMARA, Irena KRONTORÁD KOUTNÁ et. al.

Basic information

Original name

Single-cell analysis of long-range interactions of POU5F1 foci in hESC cells

Name in Czech

Single-cell analýza vzdálených interaktomů s POU5F1 v hESC liniích

Authors

STEJSKAL, Stanislav, Bára SZAROWSKÁ, Lenka TESAŘOVÁ, Pavel ŠIMARA and Irena KRONTORÁD KOUTNÁ

Edition

EMBL konference: Frontiers in Stec Cells and Cancer, 2015

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10601 Cell biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Informatics

Keywords (in Czech)

Single-cell analyza, long-range interakce, POU5F1, hESC, FISH

Keywords in English

Single-cell analysis, long-range interactions, POU5F1, hESC, FISH
Změněno: 2/3/2018 10:00, Mgr. Pavel Šimara, Ph.D.

Abstract

ORIG CZ

V originále

In the interphase nuclei, the chromosome territories are organized into the domains and chromatin loops. The chromatin loops are able to intermingle together to facilitate gene regulation in mutual nuclear sub-compartments. Stabilization of the genomic fibers in areas with increased transcription factors concentration also contributes to the high-order chromatin organization in the interphase nuclei. The communication between the chromatin regions mostly occurrs between the proximal genomic areas, but in some cases, the distal or the interchromosomal interactions were observed. Origin and the dynamics of those long-range genomic interactions are unknown. We are focused on the single cell analysis of long-range interactions with POU5F1(OCT4) gene in human embryonic stem cells (hESCs). According to the Chromatin Conformation Capture study (F. Gao, et al., Nature, 2013) we chose 5 candidates with distal interaction with POU5F1 gene. Fluorescent tagged probes (for the genomic regions that surrounded genes for POU5F1, MSX1, RARG, RUNX and TLE2) were used for visualization of genomic localization in the 3D fixed nuclei.

In Czech

In the interphase nuclei, the chromosome territories are organized into the domains and chromatin loops. The chromatin loops are able to intermingle together to facilitate gene regulation in mutual nuclear sub-compartments. Stabilization of the genomic fibers in areas with increased transcription factors concentration also contributes to the high-order chromatin organization in the interphase nuclei. The communication between the chromatin regions mostly occurrs between the proximal genomic areas, but in some cases, the distal or the interchromosomal interactions were observed. Origin and the dynamics of those long-range genomic interactions are unknown. We are focused on the single cell analysis of long-range interactions with POU5F1(OCT4) gene in human embryonic stem cells (hESCs). According to the Chromatin Conformation Capture study (F. Gao, et al., Nature, 2013) we chose 5 candidates with distal interaction with POU5F1 gene. Fluorescent tagged probes (for the genomic regions that surrounded genes for POU5F1, MSX1, RARG, RUNX and TLE2) were used for visualization of genomic localization in the 3D fixed nuclei.

Links

CZ.1.07/2.3.00/30.0030, interní kód MU
Name: Rozvoj lidských zdrojů pro oblast buněčné biologie
Investor: Ministry of Education, Youth and Sports of the CR, 2.3 Human resources in research and development
GBP302/12/G157, research and development project
Name: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii
Investor: Czech Science Foundation
MUNI/C/0993/2013, interní kód MU
Name: Regulace aktivity nádorového supresoru p53 syntetickými inhibitory CDK (Acronym: Nádorový supresor p53)
Investor: Masaryk University, Rector's Program