Detailed Information on Publication Record
2015
The melanoma-associated antigen 1 (MAGEA1) protein stimulates the E3 ubiquitin-ligase activity of TRIM31 within a TRIM31-MAGEA1-NSE4 complex
KOZÁKOVÁ, Lucie, Lucie VONDROVÁ, Karel STEJSKAL, Panagoula CHARALABOUS, Peter KOLESÁR et. al.Basic information
Original name
The melanoma-associated antigen 1 (MAGEA1) protein stimulates the E3 ubiquitin-ligase activity of TRIM31 within a TRIM31-MAGEA1-NSE4 complex
Name in Czech
Protein melanoma-associated antigen 1 (MAGEA1) stimuluje E3 ubikvitin-ligasovou aktivitu TRIM31 v rámci komplexu TRIM31-MAGEA1-NSE4
Authors
KOZÁKOVÁ, Lucie (203 Czech Republic, belonging to the institution), Lucie VONDROVÁ (203 Czech Republic, belonging to the institution), Karel STEJSKAL (203 Czech Republic, belonging to the institution), Panagoula CHARALABOUS (826 United Kingdom of Great Britain and Northern Ireland), Peter KOLESÁR (703 Slovakia, belonging to the institution), Alan R. LEHMANN (826 United Kingdom of Great Britain and Northern Ireland), Stjepan ULDRIJAN (203 Czech Republic, belonging to the institution), Christopher M. SANDERSON (826 United Kingdom of Great Britain and Northern Ireland), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution) and Jan PALEČEK (203 Czech Republic, guarantor, belonging to the institution)
Edition
Cell Cycle, Philadelphia, Taylor&Francis Inc, 2015, 1538-4101
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10601 Cell biology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 3.952
RIV identification code
RIV/00216224:14740/15:00080494
Organization unit
Central European Institute of Technology
UT WoS
000351598200022
Keywords (in Czech)
E3 ubikvitin ligasa; MAGEA1; melanoma-associated antigen; MDM4; NSE1-NSE3-NSE4 komplex; NSE4; EID proteinová rodina; evoluce proteinů; PCGF6; RING-finger proteiny; RNF166; TRIM31; TRAF6; TRIM8; TRIM41; ubikvitinace
Keywords in English
E3 ubiquitin ligase; MAGEA1; melanoma-associated antigen family; MDM4; NSE1-NSE3-NSE4 complex; NSE4; EID family; protein evolution; PCGF6; RING-finger proteins; RNF166; TRIM31; TRIM family; TRAF6; TRIM8; TRIM41; ubiquitination
Tags
International impact, Reviewed
Změněno: 13/3/2018 15:37, Mgr. Lucie Jarošová, DiS.
Abstract
V originále
The MAGE (Melanoma-associated antigen) protein family members are structurally related to each other by a MAGE-homology domain comprised of 2 winged helix motifs WH/A and WH/B. This family specifically evolved in placental mammals although single homologs designated NSE3 (non-SMC element) exist in most eukaryotes. NSE3, together with its partner proteins NSE1 and NSE4 form a tight subcomplex of the structural maintenance of chromosomes SMC5-6 complex. Previously, we showed that interactions of the WH/B motif of the MAGE proteins with their NSE4/EID partners are evolutionarily conserved (including the MAGEA1-NSE4 interaction). In contrast, the interaction of the WH/A motif of NSE3 with NSE1 diverged in the MAGE paralogs. We hypothesized that the MAGE paralogs acquired new RING-finger-containing partners through their evolution and form MAGE complexes reminiscent of NSE1-NSE3-NSE4 trimers. In this work, we employed the yeast 2-hybrid system to screen a human RING-finger protein library against several MAGE baits. We identified a number of potential MAGE-RING interactions and confirmed several of them (MDM4, PCGF6, RNF166, TRAF6, TRIM8, TRIM31, TRIM41) in co-immunoprecipitation experiments. Among these MAGE-RING pairs, we chose to examine MAGEA1-TRIM31 in detail and showed that both WH/A and WH/B motifs of MAGEA1 bind to the coiled-coil domain of TRIM31 and that MAGEA1 interaction stimulates TRIM31 ubiquitin-ligase activity. In addition, TRIM31 directly binds to NSE4, suggesting the existence of a TRIM31-MAGEA1-NSE4 complex reminiscent of the NSE1-NSE3-NSE4 trimer. These results suggest that MAGEA1 functions as a co-factor of TRIM31 ubiquitin-ligase and that the TRIM31-MAGEA1-NSE4 complex may have evolved from an ancestral NSE1-NSE3-NSE4 complex.
Links
| ED1.1.00/02.0068, research and development project |
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| EE2.3.20.0189, research and development project |
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| EE2.3.30.0037, research and development project |
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| GA13-00774S, research and development project |
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| GA14-12166S, research and development project |
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| GBP206/12/G151, research and development project |
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| IAA501630902, research and development project |
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