J 2015

Genetic Architecture of Natural Variation of Telomere Length in Arabidopsis thaliana

FULCHER, Nick, Astrid TEUBENBACHER, Envel KERDAFFREC, Ashley FARLOW, Magnus NORDBORG et. al.

Basic information

Original name

Genetic Architecture of Natural Variation of Telomere Length in Arabidopsis thaliana

Authors

FULCHER, Nick (40 Austria), Astrid TEUBENBACHER (40 Austria), Envel KERDAFFREC (40 Austria), Ashley FARLOW (40 Austria), Magnus NORDBORG (40 Austria) and Karel ŘÍHA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Genetics, BETHESDA (USA), GENETICS SOCIETY AMERICA, 2015, 0016-6731

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 4.644

RIV identification code

RIV/00216224:14740/15:00082746

Organization unit

Central European Institute of Technology

DOI

UT WoS

000349459400026

Keywords in English

telomere; QTL; centromere-mediated genome elimination; haploid; Arabidopsis

Tags

Tags

International impact, Reviewed
Změněno: 23/3/2016 10:50, Mgr. Eva Špillingová

Abstract

V originále

Telomeres represent the repetitive sequences that cap chromosome ends and are essential for their protection. Telomere length is known to be highly heritable and is derived from a homeostatic balance between telomeric lengthening and shortening activities. Specific loci that form the genetic framework underlying telomere length homeostasis, however, are not well understood. To investigate the extent of natural variation of telomere length in Arabidopsis thaliana, we examined 229 worldwide accessions by terminal restriction fragment analysis. The results showed a wide range of telomere lengths that are specific to individual accessions. To identify loci that are responsible for this variation, we adopted a quantitative trait loci (QTL) mapping approach with multiple recombinant inbred line (RIL) populations. A doubled haploid RIL population was first produced using centromere-mediated genome elimination between accessions with long (Pro-0) and intermediate (Col-0) telomere lengths. Composite interval mapping analysis of this population along with two established RIL populations (Ler-2/Cvi-0 and Est-1/Col-0) revealed a number of shared and unique QTL. QTL detected in the Ler-2/Cvi-0 population were examined using near isogenic lines that confirmed causative regions on chromosomes 1 and 2. In conclusion, this work describes the extent of natural variation of telomere length in A. thaliana, identifies a network of QTL that influence telomere length homeostasis, examines telomere length dynamics in plants with hybrid backgrounds, and shows the effects of two identified regions on telomere length regulation.