V originále
Úvod: Kolagenní matrix (collagen matrix, KM) se využívá pro augmentaci měkkých tkání v parodontologii a implantologii. Plazma bohatá na trombocyty (platelet-rich plasma, PRP) a fibrin bohatý na trombocyty (platelet-rich fibrin, PRF) jsou autologní deriváty krevní plazmy využívané v regenerativní medicíně. Cílem této experimentální intervenční kontrolované studie bylo posoudit kombinovaný efekt derivátů krevní plazmy a KM při hojení a regeneraci měkkých tkání. Materiál a metody: Ve studii jsme operovali 27 bílých novozélandských králíků. Po uvedení do celkové anestezie jsme vytvořili čtyři arteficiální defekty na sliznici horní čelisti a patra. Studované skupiny byly rozděleny náhodně (KM, KM+PRP, KM+PRF, kontrola). Defekty jsme hodnotili po 1, 7 a 28 dnech. Vyšetřující patolog hodnotil semikvantitativně akutní a chronický zánětlivý infiltrát, stupeň angiogeneze a reepitelizace rány, množství kolagenu a makrofágů. Výsledky: V žádném sledovaném parametru nebyl nalezen statisticky signifikantní rozdíl. V defektech byl přítomen nejprve akutní zánětlivý infiltrát, následně granulační tkáň a po 28 dnech byla dokončována reepitelizace. Závěr: po přidání derivátů krevní plazmy do kolagenní matrix jsme neprokázali zlepšení hojení v žádném ze sledovaných parametrů. Prohlášení: Studie byla financována z grantů MZ IGA NT14321 a MUNI/A/1359/2014. Autoři nebyli nijak podporováni žádnou společností vyrábějící zkoumané produkty.
Anglicky
Introduction: collagen matrix (CM) is used in periodontology and implant dentistry for soft tissue augmentation. Platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) are autologous blood plasma derivatives used in regenerative medicine. The aim of this experimental intervention controlled study was to evaluate the combined effect of plasma derivatives and CM in the healing and regeneration of soft tissues. Material and Methods: In this study, 27 white New Zealand rabbits were operated. After introduction of general anesthesia, four artificial defects in the mucosa of the upper jaw and palate were created. The studied groups were divided randomly (CM, CM + PRP, CM + PRF, control). The defects were evaluated after 1, 7 and 28 days.The pathologist evaluated semiquantitatively acute and chronic inflammatory infiltrate, the degree of angiogenesis and wound reepithelization, the amount of collagen and macrophages. Results: there was no statistically significant difference in any of the above mentioned parameters. The defects presented initially an acute inflammatory infiltrate, followed by granulation tissue formation and after 28 days with final stages of reepithelization. Conclusion: addition of blood plasma derivates into the collagen matrix did not improve healing in any of the monitored parameters. Introduction: collagen matrix (CM) is used in periodontology and implant dentistry for soft tissue augmentation. Platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) are autologous blood plasma derivatives used in regenerative medicine. The aim of this experimental intervention controlled study was to evaluate the combined effect of plasma derivatives and CM in the healing and regeneration of soft tissues. Material and Methods: In this study, 27 white New Zealand rabbits were operated. After introduction of general anesthesia, four artificial defects in the mucosa of the upper jaw and palate were created. The studied groups were divided randomly (CM, CM + PRP, CM + PRF, control). The defects were evaluated after 1, 7 and 28 days.The pathologist evaluated semiquantitatively acute and chronic inflammatory infiltrate, the degree of angiogenesis and wound reepithelization, the amount of collagen and macrophages. Results: there was no statistically significant difference in any of the above mentioned parameters. The defects presented initially an acute inflammatory infiltrate, followed by granulation tissue formation and after 28 days with final stages of reepithelization. Conclusion: addition of blood plasma derivates into the collagen matrix did not improve healing in any of the monitored parameters. Disclaimer: The study was funded by grants IGA MZ NT14321 and MUNI / A / 1359/2014. The authors were not supported by any company manufacturing the studied products.