Role of c-Myb in chondrogenesis

J 2015

Role of c-Myb in chondrogenesis

ORALOVÁ, Veronika, Eva MATALOVÁ, Eva JANEČKOVÁ, Eliška DROBNÁ KREJČÍ, Lucia KNOPFOVÁ et. al.

Basic information

Original name

Role of c-Myb in chondrogenesis

Name in Czech

Funkce c-Myb v chondrogenezi

Authors

ORALOVÁ, Veronika (203 Czech Republic, belonging to the institution), Eva MATALOVÁ (203 Czech Republic, guarantor), Eva JANEČKOVÁ (203 Czech Republic, belonging to the institution), Eliška DROBNÁ KREJČÍ (203 Czech Republic), Lucia KNOPFOVÁ (203 Czech Republic, belonging to the institution), Pavel ŠNAJDR (203 Czech Republic), Abigail TUCKER (826 United Kingdom of Great Britain and Northern Ireland), Iva VESELÁ (203 Czech Republic), Jan ŠMARDA (203 Czech Republic, belonging to the institution) and marcela BUCHTOVÁ (203 Czech Republic, belonging to the institution)

Edition

Bone, Elsevier, 2015, 8756-3282

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.736

RIV identification code

RIV/00216224:14310/15:00082776

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.bone.2015.02.031

UT WoS

000355718100012

Keywords (in Czech)

mikromasové kultury; myší končetiny; endochondrální kost; c-Myc; chondrogeneze

Keywords in English

micromass cultures; mouse limbs; endochondral bone; c-Myb; chondrogenesis;

Abstract

V originále

To investigate the role of c-Myb in endochondral ossification, we carried out our study on the long bones of mouse limbs during embryonic development. First, the c-myb expression pattern was analyzed by in situ hybridization during endochondral ossification of long bones. The c-Myb protein was found in proliferating chondrocytes and in the perichondrium of the forelimb bones (E14.5–E17.5). Furthermore, protein was detected in pre-hypertrophic as well as hypertrophic chondrocytes. Gain-of-function and loss-of-function approaches were used to test the effect of altered c-myb expression on chondrogenesis in micromass cultures established from forelimb buds of mouse embryos. A loss-of-function approach using c-myb specific siRNA decreased nodule formation, as well as downregulated the level of Sox9 expression, a major marker of chondrogenesis. Transient c-myb overexpression markedly increased the formation of cartilage nodules and the production of extracellular matrix as detected by intense staining with Alcian blue. Taken together, the results of this study demonstrate that the c-Myb transcription factor is involved in the regulation and promotion of endochondral bone formation.

Links

EE2.3.20.0183, research and development project

Name: Centrum experimentální biomedicíny

Citovat

ORALOVÁ, Veronika, Eva MATALOVÁ, Eva JANEČKOVÁ, Eliška DROBNÁ KREJČÍ, Lucia KNOPFOVÁ, Pavel ŠNAJDR, Abigail TUCKER, Iva VESELÁ, Jan ŠMARDA and marcela BUCHTOVÁ. Role of c-Myb in chondrogenesis. Bone. Elsevier, 2015, vol. 76, July, p. 97-106. ISSN 8756-3282. Available from: https://dx.doi.org/10.1016/j.bone.2015.02.031.

@article{1298612,
   author = {Oralová, Veronika and Matalová, Eva and Janečková, Eva and Drobná Krejčí, Eliška and Knopfová, Lucia and Šnajdr, Pavel and Tucker, Abigail and Veselá, Iva and Šmarda, Jan and Buchtová, marcela},
   article_number = {July},
   doi = {http://dx.doi.org/10.1016/j.bone.2015.02.031},
   keywords = {micromass cultures; mouse limbs; endochondral bone; c-Myb; chondrogenesis;},
   language = {eng},
   issn = {8756-3282},
   journal = {Bone},
   title = {Role of c-Myb in chondrogenesis},
   volume = {76},
   year = {2015}
}

TY  - JOUR
ID  - 1298612
AU  - Oralová, Veronika - Matalová, Eva - Janečková, Eva - Drobná Krejčí, Eliška - Knopfová, Lucia - Šnajdr, Pavel - Tucker, Abigail - Veselá, Iva - Šmarda, Jan - Buchtová, marcela
PY  - 2015
TI  - Role of c-Myb in chondrogenesis
JF  - Bone
VL  - 76
IS  - July
SP  - 97-106
EP  - 97-106
PB  - Elsevier
SN  - 87563282
KW  - micromass cultures
KW  - mouse limbs
KW  - endochondral bone
KW  - c-Myb
KW  - chondrogenesis;
N2  - To investigate the role of c-Myb in endochondral ossification, we carried out our study on the long bones of mouse limbs during embryonic development. First, the c-myb expression pattern was analyzed by in situ hybridization during endochondral ossification of long bones. The c-Myb protein was found in proliferating chondrocytes and in the perichondrium of the forelimb bones (E14.5–E17.5). Furthermore, protein was detected in pre-hypertrophic as well as hypertrophic chondrocytes. Gain-of-function and loss-of-function approaches were used to test the effect of altered c-myb expression on chondrogenesis in micromass cultures established from forelimb buds of mouse embryos. A loss-of-function approach using c-myb specific siRNA decreased nodule formation, as well as downregulated the level of Sox9 expression, a major marker of chondrogenesis. Transient c-myb overexpression markedly increased the formation of cartilage nodules and the production of extracellular matrix as detected by intense staining with Alcian blue. Taken together, the results of this study demonstrate that the c-Myb transcription factor is involved in the regulation and promotion of endochondral bone formation.
ER  -

ORALOVÁ, Veronika, Eva MATALOVÁ, Eva JANEČKOVÁ, Eliška DROBNÁ KREJČÍ, Lucia KNOPFOVÁ, Pavel ŠNAJDR, Abigail TUCKER, Iva VESELÁ, Jan ŠMARDA and marcela BUCHTOVÁ. Role of c-Myb in chondrogenesis. \textit{Bone}. Elsevier, 2015, vol.~76, July, p.~97-106. ISSN~8756-3282. Available from: https://dx.doi.org/10.1016/j.bone.2015.02.031.

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