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JUŘÍK, Tomáš, Zdeněk FARKA, David KOVÁŘ, Pavel PODEŠVA, František FORET and Petr SKLÁDAL. Gelatin templated gold nanostructured electrodes for sensitive glucose detection. In CEITEC PhD Retreat. 2015. ISBN 978-80-210-7825-3.

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TY  - CONF
ID  - 1298652
AU  - Juřík, Tomáš - Farka, Zdeněk - Kovář, David - Podešva, Pavel - Foret, František - Skládal, Petr
PY  - 2015
TI  - Gelatin templated gold nanostructured electrodes for sensitive glucose detection
SN  - 9788021078253
KW  - nanostructured electrodes
KW  - glucose detection
KW  - cyclic voltammetry
KW  - AFM
KW  - SEM
UR  - https://www.ceitec.cz/phd-retreat-abstracts/f33075
N2  - Due to the importance of glucose in physiological processes, sensitive, rapid and reliable methods for its detection are required. The biocatalytic sensors provide low limits of detection but the enzymatic layer is volatile. Sensors that are not based on enzymes are very stable in all conditions, however worse detection limits are typically obtained. One of the ways to achieve an improved sensitivity of the detection is by developing of a sensor with optimized surface morphology. In this work gelatin templated gold nanostructures were fabricated in order to improve the sensitivity of glucose detection. The 3D cavities were made by crosslinking of gelatine layer on the gold surface and nanostructured surface was formed in these cavities by electrodeposition of gold from the electroplating solution. Gel layers on the electrodes were cleaned by KOH and chromic acid. Scanning electron microscopy and atomic force microscopy were used for characterisation of the created surfaces. The glucose was detected by a direct electrochemical oxidation during cyclic voltammetry in alcaline solution. Limit of detection of 10 μM was achieved in water samples. For the detection of glucose in serum, the samples were first deproteinized in acetone, centrifugated and the pH was adjusted by KOH. The sensor was able to detect 1.3 mM glucose in diluted deproteinised human serum samples with negligible effect of interferents present in human blood serum. The results were verified using commercial glucometer ACCU-CHEK Active® and the standard kit for photometrical detection of glucose BioLaTest GLU L 500 S.
ER  -

Basic information
Original name	Gelatin templated gold nanostructured electrodes for sensitive glucose detection
Authors	JUŘÍK, Tomáš, Zdeněk FARKA, David KOVÁŘ, Pavel PODEŠVA, František FORET and Petr SKLÁDAL.
Edition	CEITEC PhD Retreat, 2015.

Other information
Original language	English
Type of outcome	Conference abstract
Field of Study	10600 1.6 Biological sciences
Country of publisher	Czech Republic
Confidentiality degree	is not subject to a state or trade secret
WWW	URL
Organization unit	Central European Institute of Technology
ISBN	978-80-210-7825-3
Keywords in English	nanostructured electrodes; glucose detection; cyclic voltammetry; AFM; SEM
Tags	International impact
Changed by	Changed by: doc. Mgr. Zdeněk Farka, Ph.D., učo 357740. Changed: 21/10/2016 15:00.

Abstract
Due to the importance of glucose in physiological processes, sensitive, rapid and reliable methods for its detection are required. The biocatalytic sensors provide low limits of detection but the enzymatic layer is volatile. Sensors that are not based on enzymes are very stable in all conditions, however worse detection limits are typically obtained. One of the ways to achieve an improved sensitivity of the detection is by developing of a sensor with optimized surface morphology. In this work gelatin templated gold nanostructures were fabricated in order to improve the sensitivity of glucose detection. The 3D cavities were made by crosslinking of gelatine layer on the gold surface and nanostructured surface was formed in these cavities by electrodeposition of gold from the electroplating solution. Gel layers on the electrodes were cleaned by KOH and chromic acid. Scanning electron microscopy and atomic force microscopy were used for characterisation of the created surfaces. The glucose was detected by a direct electrochemical oxidation during cyclic voltammetry in alcaline solution. Limit of detection of 10 μM was achieved in water samples. For the detection of glucose in serum, the samples were first deproteinized in acetone, centrifugated and the pH was adjusted by KOH. The sensor was able to detect 1.3 mM glucose in diluted deproteinised human serum samples with negligible effect of interferents present in human blood serum. The results were verified using commercial glucometer ACCU-CHEK Active® and the standard kit for photometrical detection of glucose BioLaTest GLU L 500 S.

Abstract

Due to the importance of glucose in physiological processes, sensitive, rapid and reliable methods for its detection are required. The biocatalytic sensors provide low limits of detection but the enzymatic layer is volatile. Sensors that are not based on enzymes are very stable in all conditions, however worse detection limits are typically obtained. One of the ways to achieve an improved sensitivity of the detection is by developing of a sensor with optimized surface morphology. In this work gelatin templated gold nanostructures were fabricated in order to improve the sensitivity of glucose detection. The 3D cavities were made by crosslinking of gelatine layer on the gold surface and nanostructured surface was formed in these cavities by electrodeposition of gold from the electroplating solution. Gel layers on the electrodes were cleaned by KOH and chromic acid. Scanning electron microscopy and atomic force microscopy were used for characterisation of the created surfaces. The glucose was detected by a direct electrochemical oxidation during cyclic voltammetry in alcaline solution. Limit of detection of 10 μM was achieved in water samples. For the detection of glucose in serum, the samples were first deproteinized in acetone, centrifugated and the pH was adjusted by KOH. The sensor was able to detect 1.3 mM glucose in diluted deproteinised human serum samples with negligible effect of interferents present in human blood serum. The results were verified using commercial glucometer ACCU-CHEK Active® and the standard kit for photometrical detection of glucose BioLaTest GLU L 500 S.

Links
ED1.1.00/02.0068, research and development project	Name: CEITEC - central european institute of technology