Detection of hydrogen peroxide and glucose by enzyme product
precipitation on sensor surface

J 2015

Detection of hydrogen peroxide and glucose by enzyme product precipitation on sensor surface

JUŘÍK, Tomáš and Petr SKLÁDAL

Basic information

Original name

Detection of hydrogen peroxide and glucose by enzyme product precipitation on sensor surface

Authors

JUŘÍK, Tomáš (203 Czech Republic, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Chemical papers, Berlin, WALTER DE GRUYTER GMBH, 2015, 0366-6352

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.326

RIV identification code

RIV/00216224:14750/15:00081749

Organization unit

Support Centre for Students with Special Needs

DOI

http://dx.doi.org/10.1515/chempap-2015-0003

UT WoS

000350130100015

Keywords in English

biocatalysed precipitation; horseradish peroxidase; hydrogen peroxide; 4-chloro-1-naphthol; cyclic voltammetry; quartz crystal microbalance

Abstract

V originále

Biocatalysed precipitation of an insoluble product accumulated on the enzyme-modified electrode surface was applied as the amplification path for low concentration sensing of hydrogen peroxide and glucose. Sensitive electrochemical and quartz-crystal microbalance (QCM) biosensors based on biocatalytic precipitation were developed. A horseradish peroxidase (HRP) monolayer-modified electrode was used to sense H2O2 via the oxidation of 4-chloro-1-naphthol (4CN) forming insoluble benzo-4-chlorocyclohexadienone. Additionally, the bienzyme system employed glucose oxidase (GOx) linked to HRP/4CN. The amount of the precipitate assembled on the sensing surface corresponded to the concentration of analytes and to the length of the incubation interval. The precipitated deposits were followed as a change of impedance using cyclic voltammetry (CV), mass change was determined continuously using a microgravimetric quartz-crystal microbalance, and optical microscopy enabled the visualisation of the precipitate. Regeneration of the enzyme-modified electrode was performed using cathodic reduction of the insoluble product. Thus, a simple biosensor for multiple analyses with low detection limits and of low cost can be developed. (C) 2014 Institute of Chemistry, Slovak Academy of Sciences

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

Citovat

JUŘÍK, Tomáš and Petr SKLÁDAL. Detection of hydrogen peroxide and glucose by enzyme product precipitation on sensor surface. Chemical papers. Berlin: WALTER DE GRUYTER GMBH, 2015, vol. 69, No 1, p. 167-175. ISSN 0366-6352. Available from: https://dx.doi.org/10.1515/chempap-2015-0003.

@article{1298657,
   author = {Juřík, Tomáš and Skládal, Petr},
   article_location = {Berlin},
   article_number = {1},
   doi = {http://dx.doi.org/10.1515/chempap-2015-0003},
   keywords = {biocatalysed precipitation; horseradish peroxidase; hydrogen peroxide; 4-chloro-1-naphthol; cyclic voltammetry; quartz crystal microbalance},
   language = {eng},
   issn = {0366-6352},
   journal = {Chemical papers},
   title = {Detection of hydrogen peroxide and glucose by enzyme product precipitation on sensor surface},
   volume = {69},
   year = {2015}
}

TY  - JOUR
ID  - 1298657
AU  - Juřík, Tomáš - Skládal, Petr
PY  - 2015
TI  - Detection of hydrogen peroxide and glucose by enzyme product precipitation on sensor surface
JF  - Chemical papers
VL  - 69
IS  - 1
SP  - 167-175
EP  - 167-175
PB  - WALTER DE GRUYTER GMBH
SN  - 03666352
KW  - biocatalysed precipitation
KW  - horseradish peroxidase
KW  - hydrogen peroxide
KW  - 4-chloro-1-naphthol
KW  - cyclic voltammetry
KW  - quartz crystal microbalance
N2  - Biocatalysed precipitation of an insoluble product accumulated on the enzyme-modified electrode surface was applied as the amplification path for low concentration sensing of hydrogen peroxide and glucose. Sensitive electrochemical and quartz-crystal microbalance (QCM) biosensors based on biocatalytic precipitation were developed. A horseradish peroxidase (HRP) monolayer-modified electrode was used to sense H2O2 via the oxidation of 4-chloro-1-naphthol (4CN) forming insoluble benzo-4-chlorocyclohexadienone. Additionally, the bienzyme system employed glucose oxidase (GOx) linked to HRP/4CN. The amount of the precipitate assembled on the sensing surface corresponded to the concentration of analytes and to the length of the incubation interval. The precipitated deposits were followed as a change of impedance using cyclic voltammetry (CV), mass change was determined continuously using a microgravimetric quartz-crystal microbalance, and optical microscopy enabled the visualisation of the precipitate. Regeneration of the enzyme-modified electrode was performed using cathodic reduction of the insoluble product. Thus, a simple biosensor for multiple analyses with low detection limits and of low cost can be developed. (C) 2014 Institute of Chemistry, Slovak Academy of Sciences
ER  -

JUŘÍK, Tomáš and Petr SKLÁDAL. Detection of hydrogen peroxide and glucose by enzyme product precipitation on sensor surface. \textit{Chemical papers}. Berlin: WALTER DE GRUYTER GMBH, 2015, vol.~69, No~1, p.~167-175. ISSN~0366-6352. Available from: https://dx.doi.org/10.1515/chempap-2015-0003.

Detailed Information on Publication Record