Extramedullary relapse of multiple myeloma defined as the
highest risk group based on deregulated gene expression data

J 2015

Extramedullary relapse of multiple myeloma defined as the highest risk group based on deregulated gene expression data

ŠEVČÍKOVÁ, Sabina, Helena PASZEKOVA, Lenka BEŠŠE, Lenka SEDLAŘÍKOVÁ, Veronika KUBACZKOVÁ et. al.

Basic information

Original name

Extramedullary relapse of multiple myeloma defined as the highest risk group based on deregulated gene expression data

Authors

ŠEVČÍKOVÁ, Sabina (203 Czech Republic, guarantor, belonging to the institution), Helena PASZEKOVA (203 Czech Republic), Lenka BEŠŠE (203 Czech Republic, belonging to the institution), Lenka SEDLAŘÍKOVÁ (203 Czech Republic, belonging to the institution), Veronika KUBACZKOVÁ (203 Czech Republic, belonging to the institution), Martina ALMÁŠI (203 Czech Republic, belonging to the institution), Luděk POUR (203 Czech Republic) and Roman HÁJEK (203 Czech Republic, belonging to the institution)

Edition

Biomedical Papers of the Faculty of Medicine of Palacký University, Olomouc, Czech Republic, Olomouc, Palacký University, 2015, 1213-8118

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.924

RIV identification code

RIV/00216224:14110/15:00082786

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.5507/bp.2015.014

UT WoS

000359128900020

Keywords in English

multiple myeloma; gene expression; high-risk disease; extramedullary relapse; qPCR

Abstract

V originále

Background. Multiple myeloma (MM) is characterized by malignant proliferation of plasma cells (PC) which accumulate in the bone marrow (BM). The advent of new drugs has changed the course of the disease from incurable to treatable, but most patients eventually relapse. One group of MM patients (10-15%) is considered high-risk because they relapse within 24 months. Recently, extramedullary relapse of MM (EM) has been observed more frequently. Due to its aggressivity and shorter survival, EM is also considered high-risk. Aims. The goal of this study was to determine if the so-called high-risk genes published by the University of Arkansas group (UAMS) are even more deregulated in EM patients than in high-risk MM patients and if these patients may be considered high-risk. Methods. Nine samples of bone marrow plasma cells from MM patients as well as 9 tumors and 9 bone marrow plasma cells from EM patients were used. Quantitative real-time PCR was used for evaluation of expression of 15 genes connected to the high-risk signature of MM patients. Results. Comparison of high-risk plasma cells vs extramedullary plasma cells revealed 4 significantly deregulated genes (CKS1B, CTBS, NADK, YWHAZ); moreover, comparison of extramedullary plasma cells vs extramedullary tumors revealed significant differences in 9 out of 15 genes. Of these, 6 showed significant changes as described by the UAMS group (ASPM, SLC19A1, NADK, TBRG4, TMPO and LARS2). Conclusions. Our data suggest that increasing genetic abnormalities as described by the gene expression data are associated with increased risk for EM relapse.

Links

MUNI/A/1549/2014, interní kód MU

Name: Experimentální molekulární patofyziologie vybraných komplexních chorob/ stavů (Acronym: markery komplexních nemocí)

Investor: Masaryk University, Category A

Citovat

ŠEVČÍKOVÁ, Sabina, Helena PASZEKOVA, Lenka BEŠŠE, Lenka SEDLAŘÍKOVÁ, Veronika KUBACZKOVÁ, Martina ALMÁŠI, Luděk POUR and Roman HÁJEK. Extramedullary relapse of multiple myeloma defined as the highest risk group based on deregulated gene expression data. Biomedical Papers of the Faculty of Medicine of Palacký University, Olomouc, Czech Republic. Olomouc: Palacký University, 2015, vol. 159, No 2, p. 288-293. ISSN 1213-8118. Available from: https://dx.doi.org/10.5507/bp.2015.014.

@article{1298694,
   author = {Ševčíková, Sabina and Paszekova, Helena and Bešše, Lenka and Sedlaříková, Lenka and Kubaczková, Veronika and Almáši, Martina and Pour, Luděk and Hájek, Roman},
   article_location = {Olomouc},
   article_number = {2},
   doi = {http://dx.doi.org/10.5507/bp.2015.014},
   keywords = {multiple myeloma; gene expression; high-risk disease; extramedullary relapse; qPCR},
   language = {eng},
   issn = {1213-8118},
   journal = {Biomedical Papers of the Faculty of Medicine of Palacký University, Olomouc, Czech Republic},
   title = {Extramedullary relapse of multiple myeloma defined as the highest risk group based on deregulated gene expression data},
   volume = {159},
   year = {2015}
}

TY  - JOUR
ID  - 1298694
AU  - Ševčíková, Sabina - Paszekova, Helena - Bešše, Lenka - Sedlaříková, Lenka - Kubaczková, Veronika - Almáši, Martina - Pour, Luděk - Hájek, Roman
PY  - 2015
TI  - Extramedullary relapse of multiple myeloma defined as the highest risk group based on deregulated gene expression data
JF  - Biomedical Papers of the Faculty of Medicine of Palacký University, Olomouc, Czech Republic
VL  - 159
IS  - 2
SP  - 288-293
EP  - 288-293
PB  - Palacký University
SN  - 12138118
KW  - multiple myeloma
KW  - gene expression
KW  - high-risk disease
KW  - extramedullary relapse
KW  - qPCR
N2  - Background. Multiple myeloma (MM) is characterized by malignant proliferation of plasma cells (PC) which accumulate in the bone marrow (BM). The advent of new drugs has changed the course of the disease from incurable to treatable, but most patients eventually relapse. One group of MM patients (10-15%) is considered high-risk because they relapse within 24 months. Recently, extramedullary relapse of MM (EM) has been observed more frequently. Due to its aggressivity and shorter survival, EM is also considered high-risk. Aims. The goal of this study was to determine if the so-called high-risk genes published by the University of Arkansas group (UAMS) are even more deregulated in EM patients than in high-risk MM patients and if these patients may be considered high-risk. Methods. Nine samples of bone marrow plasma cells from MM patients as well as 9 tumors and 9 bone marrow plasma cells from EM patients were used. Quantitative real-time PCR was used for evaluation of expression of 15 genes connected to the high-risk signature of MM patients. Results. Comparison of high-risk plasma cells vs extramedullary plasma cells revealed 4 significantly deregulated genes (CKS1B, CTBS, NADK, YWHAZ); moreover, comparison of extramedullary plasma cells vs extramedullary tumors revealed significant differences in 9 out of 15 genes. Of these, 6 showed significant changes as described by the UAMS group (ASPM, SLC19A1, NADK, TBRG4, TMPO and LARS2). Conclusions. Our data suggest that increasing genetic abnormalities as described by the gene expression data are associated with increased risk for EM relapse.
ER  -

ŠEVČÍKOVÁ, Sabina, Helena PASZEKOVA, Lenka BEŠŠE, Lenka SEDLAŘÍKOVÁ, Veronika KUBACZKOVÁ, Martina ALMÁŠI, Luděk POUR and Roman HÁJEK. Extramedullary relapse of multiple myeloma defined as the highest risk group based on deregulated gene expression data. \textit{Biomedical Papers of the Faculty of Medicine of Palacký University, Olomouc, Czech Republic}. Olomouc: Palacký University, 2015, vol.~159, No~2, p.~288-293. ISSN~1213-8118. Available from: https://dx.doi.org/10.5507/bp.2015.014.

Detailed Information on Publication Record