Electrochemical detection of miR-34a-5p as a promising tool for HNC and PCa diagnosis

HUDCOVÁ, Kristýna, Aneta VEČEŘOVÁ, Libuše TRNKOVÁ, Iva KEJNOVSKÁ, Michaela VORLÍČKOVÁ and Michal MASAŘÍK. Electrochemical detection of miR-34a-5p as a promising tool for HNC and PCa diagnosis. Brno: Masarykova univerzita, 2015, p. 50. ISBN 978-80-210-7825-3.

Basic information

• Original name: Electrochemical detection of miR-34a-5p as a promising tool for HNC and PCa diagnosis
• Authors: HUDCOVÁ, Kristýna, Aneta VEČEŘOVÁ, Libuše TRNKOVÁ, Iva KEJNOVSKÁ, Michaela VORLÍČKOVÁ and Michal MASAŘÍK.
• Edition: Brno, p. 50-50, 2015.
• Publisher: Masarykova univerzita

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• Publication form: printed version "print"
• Organization unit: Central European Institute of Technology
• ISBN: 978-80-210-7825-3
• Keywords (in Czech): miRNAs; rakovina hlavy a krku; rakovina prostaty; miR-34a-5p; PCR analýza; CD spektra; UV absorpční spektra; voltametrické metody
• Keywords in English: MiRNAs; head and neck cancer; prostate cancer; PCR analysis; CD spectra; UV absorption spectra; voltammetric methods

Abstract

MicroRNAs (miRNAs) are a class of non-coding RNAs that are intensively studied as biomarkers of various diseases. In this study, the attention was aimed at the research of miR-34a-5p related to head and neck cancer (HNC) and prostate cancer (PCa). MiR-34a-5p belongs to the miR-34 family and its directly linked to p53 and Wnt pathways. beside the classical molecular biology techniques of miRNA study (PCR, Northern Blotting, microarrays technologies), new biophysical approaches were applied. The results from the PCR analysis were complemented with UV absorption spectra, CD spectra and linear sweep voltammetry in connection with adsorptive stripping (AdTS) technique indicating significant differences in miR-34a-5p expression status.

Links

• CZ.1.05/1.1.00/02.0068, interní kód MU: Name: CEITEC - středoevropský technologický institut (Acronym: CEITEC)

Investor: Ministry of Education, Youth and Sports of the CR, CEITEC - Central European Institute of Technology, 1.1 European Centres of Excellence

• LH13053, research and development project: Name: Chemická a elektrochemická analýza purinových derivátů a jejich komplexů s mědí (Acronym: CECAP)

Investor: Ministry of Education, Youth and Sports of the CR

• MUNI/A/0972/2013, interní kód MU: Name: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2014 (Acronym: MIAB)

Investor: Masaryk University, Category A

• NT14337, research and development project: Name: Studium a charakterizace primárních nádorových buněčných linií spinocelulárních karcinomů v oblasti hlavy a krku a jejich maligní potenciál.

Investor: Ministry of Health of the CR