Dynamic changes in microRNA expression profiles reflect
progression of Barrett's esophagus to esophageal adenocarcinoma

J 2015

Dynamic changes in microRNA expression profiles reflect progression of Barrett's esophagus to esophageal adenocarcinoma

SLABÝ, Ondřej, Josef SROVNAL, Lenka RADOVÁ, Jan GREGAR, Jaroslav JURÁČEK et. al.

Basic information

Original name

Dynamic changes in microRNA expression profiles reflect progression of Barrett's esophagus to esophageal adenocarcinoma

Authors

SLABÝ, Ondřej (203 Czech Republic, guarantor, belonging to the institution), Josef SROVNAL (203 Czech Republic), Lenka RADOVÁ (203 Czech Republic, belonging to the institution), Jan GREGAR (203 Czech Republic), Jaroslav JURÁČEK (203 Czech Republic, belonging to the institution), Pavla LUŽNÁ (203 Czech Republic), Marek SVOBODA (203 Czech Republic, belonging to the institution), Marian HAJDÚCH (203 Czech Republic) and Jiří EHRAMANN (203 Czech Republic)

Edition

Carcinogenesis, Oxford, Oxford University Press, 2015, 0143-3334

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.874

RIV identification code

RIV/00216224:14740/15:00080394

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1093/carcin/bgv023

UT WoS

000354746400002

Keywords in English

microRNA; Barrett's esophagus; esophageal carcinoma

Abstract

V originále

Esophageal adenocarcinoma (EAC) is highly aggressive malignancy that frequently develops from Barrett's esophagus (BE), a premalignant pathologic change occurring in the lower end of the esophagus. MicroRNAs (miRNAs) are small, non-coding RNAs that function as posttranscriptional regulators of gene expression and were repeatedly proved to play key roles in pathogenesis of BE as well as EAC. In our study, we used Affymetrix GeneChip miRNA arrays to obtain miRNA expression profiles in total of 119 tissue samples [24 normal esophageal mucosa (EM), 60 BE and 35 EAC]. We identified a number of miRNAs, that showed altered expression progressively in sequence EM, BE and EAC, including for instance miR-21, miR-25, miR-194 and miR-196a with increasing levels (P < 0.0015) and miR-203, miR-205, miR-210 and miR-378 with decreasing levels (P < 0.0001). The subsequent analysis revealed four diagnostic miRNA signatures enabling to distinguish EM and BE [12 miRNAs, area under curve (AUC) = 0.971], EM and EAC (13 miRNAs, AUC = 1.0), BE without and BE with dysplasia (21 miRNAs, AUC = 0.856) and BE without dysplastic changes and BE with dysplasia together with EAC (2 miRNAs, AUC = 0.886). We suggest that miRNA expression profiling expands current knowledge in molecular pathology of Barrett's-based carcinogenesis and enables identification of molecular biomarkers for early detection of BE dysplasia and progression to EAC.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

TE02000058, research and development project

Name: Centrum kompetence pro molekulární diagnostiku a personalizovanou medicínu (Acronym: MOLDIMED)

Investor: Technology Agency of the Czech Republic

Citovat

SLABÝ, Ondřej, Josef SROVNAL, Lenka RADOVÁ, Jan GREGAR, Jaroslav JURÁČEK, Pavla LUŽNÁ, Marek SVOBODA, Marian HAJDÚCH and Jiří EHRAMANN. Dynamic changes in microRNA expression profiles reflect progression of Barrett's esophagus to esophageal adenocarcinoma. Carcinogenesis. Oxford: Oxford University Press, 2015, vol. 36, No 5, p. 521-527. ISSN 0143-3334. Available from: https://dx.doi.org/10.1093/carcin/bgv023.

@article{1298918,
   author = {Slabý, Ondřej and Srovnal, Josef and Radová, Lenka and Gregar, Jan and Juráček, Jaroslav and Lužná, Pavla and Svoboda, Marek and Hajdúch, Marian and Ehramann, Jiří},
   article_location = {Oxford},
   article_number = {5},
   doi = {http://dx.doi.org/10.1093/carcin/bgv023},
   keywords = {microRNA; Barrett's esophagus; esophageal carcinoma},
   language = {eng},
   issn = {0143-3334},
   journal = {Carcinogenesis},
   title = {Dynamic changes in microRNA expression profiles reflect progression of Barrett's esophagus to esophageal adenocarcinoma},
   url = {http://carcin.oxfordjournals.org/content/36/5/521},
   volume = {36},
   year = {2015}
}

TY  - JOUR
ID  - 1298918
AU  - Slabý, Ondřej - Srovnal, Josef - Radová, Lenka - Gregar, Jan - Juráček, Jaroslav - Lužná, Pavla - Svoboda, Marek - Hajdúch, Marian - Ehramann, Jiří
PY  - 2015
TI  - Dynamic changes in microRNA expression profiles reflect progression of Barrett's esophagus to esophageal adenocarcinoma
JF  - Carcinogenesis
VL  - 36
IS  - 5
SP  - 521-527
EP  - 521-527
PB  - Oxford University Press
SN  - 01433334
KW  - microRNA
KW  - Barrett's esophagus
KW  - esophageal carcinoma
UR  - http://carcin.oxfordjournals.org/content/36/5/521
L2  - http://carcin.oxfordjournals.org/content/36/5/521
N2  - Esophageal adenocarcinoma (EAC) is highly aggressive malignancy that frequently develops from Barrett's esophagus (BE), a premalignant pathologic change occurring in the lower end of the esophagus. MicroRNAs (miRNAs) are small, non-coding RNAs that function as posttranscriptional regulators of gene expression and were repeatedly proved to play key roles in pathogenesis of BE as well as EAC. In our study, we used Affymetrix GeneChip miRNA arrays to obtain miRNA expression profiles in total of 119 tissue samples [24 normal esophageal mucosa (EM), 60 BE and 35 EAC]. We identified a number of miRNAs, that showed altered expression progressively in sequence EM, BE and EAC, including for instance miR-21, miR-25, miR-194 and miR-196a with increasing levels (P < 0.0015) and miR-203, miR-205, miR-210 and miR-378 with decreasing levels (P < 0.0001). The subsequent analysis revealed four diagnostic miRNA signatures enabling to distinguish EM and BE [12 miRNAs, area under curve (AUC) = 0.971], EM and EAC (13 miRNAs, AUC = 1.0), BE without and BE with dysplasia (21 miRNAs, AUC = 0.856) and BE without dysplastic changes and BE with dysplasia together with EAC (2 miRNAs, AUC = 0.886). We suggest that miRNA expression profiling expands current knowledge in molecular pathology of Barrett's-based carcinogenesis and enables identification of molecular biomarkers for early detection of BE dysplasia and progression to EAC.
ER  -

SLABÝ, Ondřej, Josef SROVNAL, Lenka RADOVÁ, Jan GREGAR, Jaroslav JURÁČEK, Pavla LUŽNÁ, Marek SVOBODA, Marian HAJDÚCH and Jiří EHRAMANN. Dynamic changes in microRNA expression profiles reflect progression of Barrett's esophagus to esophageal adenocarcinoma. \textit{Carcinogenesis}. Oxford: Oxford University Press, 2015, vol.~36, No~5, p.~521-527. ISSN~0143-3334. Available from: https://dx.doi.org/10.1093/carcin/bgv023.

Detailed Information on Publication Record