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@article{1299018,
author = {Krejčí, Jana and Stixová, Lenka and Pagáčová, Eva and Legartová, Soňa and Kozubek, Stanislav and Lochmanová, Gabriela and Zdráhal, Zbyněk and Sehnalová, Petra and Dabravolski, Siarhei and Hejátko, Jan and Bártová, Eva},
article_location = {Hoboken (USA)},
article_number = {10},
doi = {http://dx.doi.org/10.1002/jcb.25170},
keywords = {HUMAN SPERM; HISTONES; PROTAMINE P2; EPIGENETICS; MICROSCOPY},
language = {eng},
issn = {0730-2312},
journal = {Journal of cellular biochemistry},
title = {Post-Translational Modifications of Histones in Human Sperm},
url = {http://onlinelibrary.wiley.com/doi/10.1002/jcb.25170/pdf},
volume = {116},
year = {2015}
}
TY - JOUR
ID - 1299018
AU - Krejčí, Jana - Stixová, Lenka - Pagáčová, Eva - Legartová, Soňa - Kozubek, Stanislav - Lochmanová, Gabriela - Zdráhal, Zbyněk - Sehnalová, Petra - Dabravolski, Siarhei - Hejátko, Jan - Bártová, Eva
PY - 2015
TI - Post-Translational Modifications of Histones in Human Sperm
JF - Journal of cellular biochemistry
VL - 116
IS - 10
SP - 2195-2209
EP - 2195-2209
PB - WILEY-BLACKWELL
SN - 07302312
KW - HUMAN SPERM
KW - HISTONES
KW - PROTAMINE P2
KW - EPIGENETICS
KW - MICROSCOPY
UR - http://onlinelibrary.wiley.com/doi/10.1002/jcb.25170/pdf
L2 - http://onlinelibrary.wiley.com/doi/10.1002/jcb.25170/pdf
N2 - We examined the levels and distribution of post-translationally modified histones and protamines in human sperm. Using western blot immunoassay, immunofluorescence, mass spectrometry (MS), and FLIM-FRET approaches, we analyzed the status of histone modifications and the protamine P2. Among individual samples, we observed variability in the levels of H3K9me1, H3K9me2, H3K27me3, H3K36me3, and H3K79me1, but the level of acetylated (ac) histones H4 was relatively stable in the sperm head fractions, as demonstrated by western blot analysis. Sperm heads with lower levels of P2 exhibited lower levels of H3K9ac, H3K9me1, H3K27me3, H3K36me3, and H3K79me1. A very strong correlation was observed between the levels of P2 and H3K9me2. FLIM-FRET analysis additionally revealed that acetylated histones H4 are not only parts of sperm chromatin but also appear in a non-integrated form. Intriguingly, H4ac and H3K27me3 were detected in sperm tail fractions via western blot analysis. An appearance of specific histone H3 and H4 acetylation and H3 methylation in sperm tail fractions was also confirmed by both LC-MS/MS and MALDI-TOF MS analysis. Taken together, these data indicate that particular post-translational modifications of histones are uniquely distributed in human sperm, and this distribution varies among individuals and among the sperm of a single individual.
ER -
KREJČÍ, Jana, Lenka STIXOVÁ, Eva PAGÁČOVÁ, Soňa LEGARTOVÁ, Stanislav KOZUBEK, Gabriela LOCHMANOVÁ, Zbyněk ZDRÁHAL, Petra SEHNALOVÁ, Siarhei DABRAVOLSKI, Jan HEJÁTKO a Eva BÁRTOVÁ. Post-Translational Modifications of Histones in Human Sperm. \textit{Journal of cellular biochemistry}. Hoboken (USA): WILEY-BLACKWELL, roč.~116, č.~10, s.~2195-2209. ISSN~0730-2312. doi:10.1002/jcb.25170. 2015.
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