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@article{1299079,
author = {Kopecká, Jana and Němec, Miroslav and Matoulková, Dagmar and Čejka, Pavel and Jelínková, Markéta and Felsberg, Jürgen and Sigler, Karel},
article_number = {2},
doi = {http://dx.doi.org/10.1094/ASBCJ-2015-0324-01},
keywords = {Ale and lager yeast Cell surface hydrophobicity FLO genes Flocculation Helm’s test},
language = {eng},
issn = {0361-0470},
journal = {The Journal of the American Society of Brewing Chemists},
title = {Effect of Growth Conditions on Flocculation and Cell Surface Hydrophobicity of Brewing Yeast},
volume = {73},
year = {2015}
}
TY - JOUR
ID - 1299079
AU - Kopecká, Jana - Němec, Miroslav - Matoulková, Dagmar - Čejka, Pavel - Jelínková, Markéta - Felsberg, Jürgen - Sigler, Karel
PY - 2015
TI - Effect of Growth Conditions on Flocculation and Cell Surface Hydrophobicity of Brewing Yeast
JF - The Journal of the American Society of Brewing Chemists
VL - 73
IS - 2
SP - 143-150
EP - 143-150
PB - American Society of Brewing Chemists
SN - 03610470
KW - Ale and lager yeast Cell surface hydrophobicity FLO genes Flocculation Helm’s test
N2 - Flocculation and cell surface hydrophobicity (CSH) were determined in anaerobic, static, and aerobic shaken stationary (72- and 96-h growth) cultures of two lager and two ale brewing yeast strains in YPD medium and in 12 and 18°P all-malt worts at 30°C. Ale and lager strains showed no substantial differences in the number and distribution of their FLO genes. In cultures grown anaerobically under CO2, both flocculation and CSH were high and very similar (81.26 ± 4.61% flocculation and 82.07 ± 4.09% CSH) regardless of culture medium and yeast strain. Strain- and medium-specific differences appeared only when the cultures were supplied with oxygen. In static cultures, flocculation was somewhat higher in lager strains (87.76 ± 3.07%) than in ale strains (81.26 ± 5.09%); in aerobic cultures, the difference was even higher (lager 58.69 ± 7.96%; ale 39.90 ± 9.24%). Flocculation in static YPD- and wort-grown lager cultures was similar, while in ale strains it was always higher in wort-grown cultures depending on the wort gravity. In aerobic cultures, flocculation was, in general, lower than in static cultures, and it was strongly reduced in wort-grown cultures. Similar to flocculation, CSH in both static and aerobic cultures was strain-dependent, always being higher in lager strains. Furthermore, in static YPD-grown cells, CSH was lower than in wort-grown cultures, while in aerobic cultures, it was higher. Correlation between flocculation and CSH, which was missing in anaerobic cultures, was strong in lager yeast strains in both static and aerobic shaken ones. Strong correlation was found between lager and ale yeast strains in both 12 and 18°P worts, while a relationship was missing after growth in YPD.
ER -
KOPECKÁ, Jana, Miroslav NĚMEC, Dagmar MATOULKOVÁ, Pavel ČEJKA, Markéta JELÍNKOVÁ, Jürgen FELSBERG and Karel SIGLER. Effect of Growth Conditions on Flocculation and Cell Surface Hydrophobicity of Brewing Yeast. \textit{The Journal of the American Society of Brewing Chemists}. American Society of Brewing Chemists, 2015, vol.~73, No~2, p.~143-150. ISSN~0361-0470. Available from: https://dx.doi.org/10.1094/ASBCJ-2015-0324-01.
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