Protococcidian Eleutheroschizon duboscqi, an unusual
apicomplexan interconnecting gregarines and cryptosporidia.

J 2015

Protococcidian Eleutheroschizon duboscqi, an unusual apicomplexan interconnecting gregarines and cryptosporidia.

VALIGUROVÁ, Andrea, Gita G. PASKEROVA, Andrei DIAKIN, Magdaléna KOVÁČIKOVÁ, Timur G. SIMDYANOV et. al.

Basic information

Original name

Protococcidian Eleutheroschizon duboscqi, an unusual apicomplexan interconnecting gregarines and cryptosporidia.

Authors

VALIGUROVÁ, Andrea (703 Slovakia, guarantor, belonging to the institution), Gita G. PASKEROVA (643 Russian Federation), Andrei DIAKIN (643 Russian Federation, belonging to the institution), Magdaléna KOVÁČIKOVÁ (703 Slovakia, belonging to the institution) and Timur G. SIMDYANOV (643 Russian Federation)

Edition

PLOS ONE, Public Library of Science, 2015, 1932-6203

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.057

RIV identification code

RIV/00216224:14310/15:00080794

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1371/journal.pone.0125063

UT WoS

000353659100093

Keywords in English

actin; Apicomplexa; attachment; coccidia; Cryptosporidium; Eleutheroschizon duboscqi; gregarines; myosin; tubulin; ultrastructure

Abstract

V originále

Study focuses on the attachment strategy, cell structure and the host-parasite interactions of the protococcidian Eleutheroschizon duboscqi, parasitising the polychaete Scoloplos armiger. The attached trophozoites and gamonts of E. duboscqi were detected at different development stages. They develop epicellularly, covered by a host cell-derived parasitophorous sac forming a caudal tipped appendage. Evans blue staining suggests that this tail is protein-rich, supported by the presence of a fibrous substance in this area. Despite the ultrastructural evidence for long filaments in the tail, it stained only weakly for F-actin, while spectrin seemed to accumulate in this area. Attachment apparatus consists of lobes arranged in one (trophozoites) or two (gamonts) circles, crowned by a ring of filamentous fascicles. During trophozoite maturation, the internal space between the parasitophorous sac and parasite turns translucent, the parasite trilaminar pellicle seems to reorganise and is covered by a dense fibrous glycocalyx. The parasite surface is organised in broad folds with grooves in between. Micropores are situated at the bottom of the grooves. A layer of filaments organised in bands, underlying the folds and ending above the attachment fascicles, was detected just beneath the pellicle. Confocal microscopy, along with the application of cytoskeletal drugs confirmed the presence of actin and tubulin polymerised forms in both the parasitophorous sac and the parasite, while myosin labelling was restricted to the sac. Despite positive tubulin labelling, no microtubules were detected in mature stages. The attachment strategy of E. duboscqi shares features with that of cryptosporidia and gregarines, i.e. the parasite itself conspicuously resembles an epicellularly located gregarine, while the parasitophorous sac develops in a similar manner to that in cryptosporidia. Study provides a re-evaluation of epicellular development in apicomplexans.

Links

GBP505/12/G112, research and development project

Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation

Citovat

VALIGUROVÁ, Andrea, Gita G. PASKEROVA, Andrei DIAKIN, Magdaléna KOVÁČIKOVÁ and Timur G. SIMDYANOV. Protococcidian Eleutheroschizon duboscqi, an unusual apicomplexan interconnecting gregarines and cryptosporidia. PLOS ONE. Public Library of Science, 2015, vol. 10, No 4, p. "nestránkováno", 27 pp. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0125063.

@article{1299247,
   author = {Valigurová, Andrea and Paskerova, Gita G. and Diakin, Andrei and Kováčiková, Magdaléna and Simdyanov, Timur G.},
   article_number = {4},
   doi = {http://dx.doi.org/10.1371/journal.pone.0125063},
   keywords = {actin; Apicomplexa; attachment; coccidia; Cryptosporidium; Eleutheroschizon duboscqi; gregarines; myosin; tubulin; ultrastructure},
   language = {eng},
   issn = {1932-6203},
   journal = {PLOS ONE},
   title = {Protococcidian Eleutheroschizon duboscqi, an unusual apicomplexan interconnecting gregarines and cryptosporidia.},
   url = {http://www.plosone.org/article/Authors/info:doi/10.1371/journal.pone.0125063},
   volume = {10},
   year = {2015}
}

TY  - JOUR
ID  - 1299247
AU  - Valigurová, Andrea - Paskerova, Gita G. - Diakin, Andrei - Kováčiková, Magdaléna - Simdyanov, Timur G.
PY  - 2015
TI  - Protococcidian Eleutheroschizon duboscqi, an unusual apicomplexan interconnecting gregarines and cryptosporidia.
JF  - PLOS ONE
VL  - 10
IS  - 4
SP  - "nestránkováno"
EP  - "nestránkováno"
PB  - Public Library of Science
SN  - 19326203
KW  - actin
KW  - Apicomplexa
KW  - attachment
KW  - coccidia
KW  - Cryptosporidium
KW  - Eleutheroschizon duboscqi
KW  - gregarines
KW  - myosin
KW  - tubulin
KW  - ultrastructure
UR  - http://www.plosone.org/article/Authors/info:doi/10.1371/journal.pone.0125063
L2  - http://www.plosone.org/article/Authors/info:doi/10.1371/journal.pone.0125063
N2  - Study focuses on the attachment strategy, cell structure and the host-parasite interactions of the protococcidian Eleutheroschizon duboscqi, parasitising the polychaete Scoloplos armiger. The attached trophozoites and gamonts of E. duboscqi were detected at different development stages. They develop epicellularly, covered by a host cell-derived parasitophorous sac forming a caudal tipped appendage. Evans blue staining suggests that this tail is protein-rich, supported by the presence of a fibrous substance in this area. Despite the ultrastructural evidence for long filaments in the tail, it stained only weakly for F-actin, while spectrin seemed to accumulate in this area. Attachment apparatus consists of lobes arranged in one (trophozoites) or two (gamonts) circles, crowned by a ring of filamentous fascicles. During trophozoite maturation, the internal space between the parasitophorous sac and parasite turns translucent, the parasite trilaminar pellicle seems to reorganise and is covered by a dense fibrous glycocalyx. The parasite surface is organised in broad folds with grooves in between. Micropores are situated at the bottom of the grooves. A layer of filaments organised in bands, underlying the folds and ending above the attachment fascicles, was detected just beneath the pellicle. Confocal microscopy, along with the application of cytoskeletal drugs confirmed the presence of actin and tubulin polymerised forms in both the parasitophorous sac and the parasite, while myosin labelling was restricted to the sac. Despite positive tubulin labelling, no microtubules were detected in mature stages. The attachment strategy of E. duboscqi shares features with that of cryptosporidia and gregarines, i.e. the parasite itself conspicuously resembles an epicellularly located gregarine, while the parasitophorous sac develops in a similar manner to that in cryptosporidia. Study provides a re-evaluation of epicellular development in apicomplexans.
ER  -

VALIGUROVÁ, Andrea, Gita G. PASKEROVA, Andrei DIAKIN, Magdaléna KOVÁČIKOVÁ and Timur G. SIMDYANOV. Protococcidian Eleutheroschizon duboscqi, an unusual apicomplexan interconnecting gregarines and cryptosporidia. \textit{PLOS ONE}. Public Library of Science, 2015, vol.~10, No~4, p.~''nestránkováno'', 27 pp. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0125063.

Detailed Information on Publication Record