Detailed Information on Publication Record
2015
Protococcidian Eleutheroschizon duboscqi, an unusual apicomplexan interconnecting gregarines and cryptosporidia.
VALIGUROVÁ, Andrea, Gita G. PASKEROVA, Andrei DIAKIN, Magdaléna KOVÁČIKOVÁ, Timur G. SIMDYANOV et. al.Basic information
Original name
Protococcidian Eleutheroschizon duboscqi, an unusual apicomplexan interconnecting gregarines and cryptosporidia.
Authors
VALIGUROVÁ, Andrea (703 Slovakia, guarantor, belonging to the institution), Gita G. PASKEROVA (643 Russian Federation), Andrei DIAKIN (643 Russian Federation, belonging to the institution), Magdaléna KOVÁČIKOVÁ (703 Slovakia, belonging to the institution) and Timur G. SIMDYANOV (643 Russian Federation)
Edition
PLOS ONE, Public Library of Science, 2015, 1932-6203
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 3.057
RIV identification code
RIV/00216224:14310/15:00080794
Organization unit
Faculty of Science
UT WoS
000353659100093
Keywords in English
actin; Apicomplexa; attachment; coccidia; Cryptosporidium; Eleutheroschizon duboscqi; gregarines; myosin; tubulin; ultrastructure
Tags
Actin, AKR, Apicomplexa, attachment, attachment site, Biology, Cryptosporidium, development, epicellular, Epicellular localisation, Eugregarine trophozoite, gregarines, host cell invasion, invertebrates, morphology, myosin, parasitophorous sac, rivok, scanning electron microscopy, tubulin, ultrastructure
Tags
International impact, Reviewed
Změněno: 16/2/2018 14:28, doc. RNDr. Andrea Bardůnek Valigurová, Ph.D.
Abstract
V originále
Study focuses on the attachment strategy, cell structure and the host-parasite interactions of the protococcidian Eleutheroschizon duboscqi, parasitising the polychaete Scoloplos armiger. The attached trophozoites and gamonts of E. duboscqi were detected at different development stages. They develop epicellularly, covered by a host cell-derived parasitophorous sac forming a caudal tipped appendage. Evans blue staining suggests that this tail is protein-rich, supported by the presence of a fibrous substance in this area. Despite the ultrastructural evidence for long filaments in the tail, it stained only weakly for F-actin, while spectrin seemed to accumulate in this area. Attachment apparatus consists of lobes arranged in one (trophozoites) or two (gamonts) circles, crowned by a ring of filamentous fascicles. During trophozoite maturation, the internal space between the parasitophorous sac and parasite turns translucent, the parasite trilaminar pellicle seems to reorganise and is covered by a dense fibrous glycocalyx. The parasite surface is organised in broad folds with grooves in between. Micropores are situated at the bottom of the grooves. A layer of filaments organised in bands, underlying the folds and ending above the attachment fascicles, was detected just beneath the pellicle. Confocal microscopy, along with the application of cytoskeletal drugs confirmed the presence of actin and tubulin polymerised forms in both the parasitophorous sac and the parasite, while myosin labelling was restricted to the sac. Despite positive tubulin labelling, no microtubules were detected in mature stages. The attachment strategy of E. duboscqi shares features with that of cryptosporidia and gregarines, i.e. the parasite itself conspicuously resembles an epicellularly located gregarine, while the parasitophorous sac develops in a similar manner to that in cryptosporidia. Study provides a re-evaluation of epicellular development in apicomplexans.
Links
| GBP505/12/G112, research and development project |
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