a 2015

Motility of archigregarine Selenidium sp. parasitizing polychaete Pygospio elegans from White Sea

KOVÁČIKOVÁ, Magdaléna, Andrei DIAKIN, Gita G. PASKEROVA and Andrea VALIGUROVÁ

Basic information

Original name

Motility of archigregarine Selenidium sp. parasitizing polychaete Pygospio elegans from White Sea

Authors

KOVÁČIKOVÁ, Magdaléna (703 Slovakia, guarantor, belonging to the institution), Andrei DIAKIN (643 Russian Federation, belonging to the institution), Gita G. PASKEROVA (643 Russian Federation) and Andrea VALIGUROVÁ (703 Slovakia, belonging to the institution)

Edition

Students In Polar Research Conference, 2015

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/15:00080796

Organization unit

Faculty of Science

Keywords (in Czech)

Apicomplexa; Gregarine; Motility; Parasites; White Sea

Keywords in English

Apicomplexa; Gregarine; Motility; Parasites; White Sea
Změněno: 13/5/2015 09:50, Mgr. Magdaléna Kováčiková, Ph.D.

Abstract

V originále

Phylum Apicomplexa belongs to the most monitored group of protists, comprising exclusively parasites of vertebrates and invertebrates. The phylum also includes gregarines – relatively large parasites inhabiting intestine, body cavity or different tissue of invertebrates. It is known that marine gregarines (mainly archigregarines) possess specific characteristics inferred to be ancestral for the phylum. Among theories on apicomplexan motility, the 'glideosome' concept, first announced for Toxoplasma gondii takes the principal place. Nevertheless, the exact mechanism of motility still remains unknown in early emerging groups of Apicomplexa, comprising gregarines. Movement in these organisms usually differs from the typical substrate dependent gliding and seem it correlate with various modifications of their cell cortex. Here, we present our preliminary data onthe motility of an archigregarine Selenidium sp. parasitizing the intestine of marine arctic polychaete Pygospio elegans. In general, gregarines of genus Selenidium exhibit pendular or rolling movement.Their cell surface displays broad longitudinal folds separated by grooves. Beneath each fold, sets of parallel longitudinal subpellicular microtubules are present. In this study, we performed experiments on living parasites treated with cytoskeletal drugs, to determine the role of actin filaments and subpellicular microtubules in their motility. For monitoring the actin filaments,two probes were used: jasplakinolide (induces actin polymerisation) and cytochalasineD (inhibits actin polymerisation). To verify the role of subpellicular microtubules in parasite movement, oryzalin and colchicine were used. The changes of gregarine motility induced by drugs were monitored under light microscopy and processed for further immunofluorescent and electron microscopic analyses. Comprehension of cell motility, especially in ancestral lineages of apicomplexans, appears important for controlling diseases and for their treatment.

Links

GBP505/12/G112, research and development project
Name: ECIP - Evropské centrum ichtyoparazitologie
Investor: Czech Science Foundation