FARKA, Zdeněk, David KOVÁŘ and Petr SKLÁDAL. Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms. Online. In Augustus Way Fountain. Proc. SPIE 9455, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XVI. USA: SPIE, 2015, p. 1-9. ISBN 978-1-62841-571-1. Available from: https://dx.doi.org/10.1117/12.2084606.
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Basic information
Original name Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms
Authors FARKA, Zdeněk (203 Czech Republic, belonging to the institution), David KOVÁŘ (203 Czech Republic, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution).
Edition USA, Proc. SPIE 9455, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XVI, p. 1-9, 9 pp. 2015.
Publisher SPIE
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 10600 1.6 Biological sciences
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Publication form electronic version available online
WWW URL
RIV identification code RIV/00216224:14740/15:00082954
Organization unit Central European Institute of Technology
ISBN 978-1-62841-571-1
ISSN 0277-786X
Doi http://dx.doi.org/10.1117/12.2084606
UT WoS 000357744700006
Keywords in English Biosensor; Cyclone sampling; Quartz crystal microbalance; Bioaerosol; Escherichia coli
Tags International impact, Reviewed
Changed by Changed by: doc. Mgr. Zdeněk Farka, Ph.D., učo 357740. Changed: 11/8/2015 08:59.
Abstract
Biological warfare agents (BWAs) represent the current menace of the asymmetric war. The early detection of BWAs, especially in the form of bioaerosol, is a challenging task for governments all around the world. Label-free quartz crystal microbalance (QCM) immunosensor and electrochemical immunosensor were developed and tested for rapid detection of BWA surrogate (E. coli) in the form of bioaerosol. Two immobilization strategies for the attachment of antibody were tested; the gold sensor surface was activated by cysteamine and then antibody was covalently linked either using glutaraldehyde, or the reduced antibodies were attached via Sulfo-SMCC. A portable bioaerosol chamber was constructed and used for safe manipulation with aerosolized microorganisms. The dissemination was done using a piezoelectric humidifier, distribution of bioaerosol inside the chamber was ensured using three 12-cm fans. The whole system was controlled remotely using LAN network. The disseminated microbial cells were collected and preconcentrated using the wetted-wall cyclone SASS 2300, the analysis was done using the on-line linked immunosensors. The QCM immunosensor had limit of detection 1×10^4 CFU·L-1 of air with analysis time 16 min, the whole experiment including dissemination and sensor surface regeneration took 40 min. In case of blank (disseminated sterile buffer), no signal change was observed. The electrochemical immunosensor was able to detect 150 CFU·L-1 of air in 20 min; also in this case, no interferences were observed. Reference measurements were done using particle counter Met One 3400 and by cultivation method on agar plates. The sensors have proved to be applicable for rapid screening of microorganisms in air.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
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