Site-specific Analysis of Protein Hydration Based on Unnatural
Amino Acid Fluorescence.

J 2015

Site-specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence.

AMARO, M., Jan BREZOVSKÝ, Silvia KOVÁČOVÁ, J. SYKORA, David BEDNÁŘ et. al.

Basic information

Original name

Site-specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence.

Authors

AMARO, M. (620 Portugal), Jan BREZOVSKÝ (203 Czech Republic, belonging to the institution), Silvia KOVÁČOVÁ (703 Slovakia, belonging to the institution), J. SYKORA (203 Czech Republic), David BEDNÁŘ (203 Czech Republic, belonging to the institution), Václav NĚMEC (203 Czech Republic, belonging to the institution), Veronika LIŠKOVÁ (203 Czech Republic, belonging to the institution), Nagendra Prasad KURUMBANG (524 Nepal, belonging to the institution), Koen BEERENS (56 Belgium, belonging to the institution), Radka CHALOUPKOVÁ (203 Czech Republic, belonging to the institution), Kamil PARUCH (203 Czech Republic, belonging to the institution), Martina HOF (276 Germany) and Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of the American Chemical Society, 2015, 0002-7863

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 13.038

RIV identification code

RIV/00216224:14310/15:00080814

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1021/jacs.5b01681

UT WoS

000353606700029

Keywords in English

dehalogenases;fluorescence spectroscopy;molecular dynamics simulations

Abstract

V originále

Hydration of proteins profoundly affects their functions. We describe a simple and general method for site-specific analysis of protein hydration based on the in vivo incorporation of fluorescent unnatural amino acids and their analysis by steady-state fluorescence spectroscopy. Using this method, we investigate the hydration of functionally important regions of dehalogenases. The experimental results are compared to findings from molecular dynamics simulations.

Links

EE2.3.30.0037, research and development project

Name: Zaměstnáním nejlepších mladých vědců k rozvoji mezinárodní spolupráce

GAP207/12/0775, research and development project

Name: Strukturně-funkční vztahy haloalkan dehalogenas

Investor: Czech Science Foundation

LM2010005, research and development project

Name: Velká infrastruktura CESNET (Acronym: VI CESNET)

Investor: Ministry of Education, Youth and Sports of the CR

LO1214, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

AMARO, M., Jan BREZOVSKÝ, Silvia KOVÁČOVÁ, J. SYKORA, David BEDNÁŘ, Václav NĚMEC, Veronika LIŠKOVÁ, Nagendra Prasad KURUMBANG, Koen BEERENS, Radka CHALOUPKOVÁ, Kamil PARUCH, Martina HOF and Jiří DAMBORSKÝ. Site-specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence. Journal of the American Chemical Society. 2015, vol. 137, No 15, p. 4988-4992. ISSN 0002-7863. Available from: https://dx.doi.org/10.1021/jacs.5b01681.

@article{1300082,
   author = {Amaro, M. and Brezovský, Jan and Kováčová, Silvia and Sykora, J. and Bednář, David and Němec, Václav and Lišková, Veronika and Kurumbang, Nagendra Prasad and Beerens, Koen and Chaloupková, Radka and Paruch, Kamil and Hof, Martina and Damborský, Jiří},
   article_number = {15},
   doi = {http://dx.doi.org/10.1021/jacs.5b01681},
   keywords = {dehalogenases;fluorescence spectroscopy;molecular dynamics simulations},
   language = {eng},
   issn = {0002-7863},
   journal = {Journal of the American Chemical Society},
   title = {Site-specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence.},
   url = {http://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2015/04/jacs15.pdf},
   volume = {137},
   year = {2015}
}

TY  - JOUR
ID  - 1300082
AU  - Amaro, M. - Brezovský, Jan - Kováčová, Silvia - Sykora, J. - Bednář, David - Němec, Václav - Lišková, Veronika - Kurumbang, Nagendra Prasad - Beerens, Koen - Chaloupková, Radka - Paruch, Kamil - Hof, Martina - Damborský, Jiří
PY  - 2015
TI  - Site-specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence.
JF  - Journal of the American Chemical Society
VL  - 137
IS  - 15
SP  - 4988-4992
EP  - 4988-4992
SN  - 00027863
KW  - dehalogenases;fluorescence spectroscopy;molecular dynamics simulations
UR  - http://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2015/04/jacs15.pdf
L2  - http://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2015/04/jacs15.pdf
N2  - Hydration of proteins profoundly affects their functions. We describe a simple and general method for site-specific analysis of protein hydration based on the in vivo incorporation of fluorescent unnatural amino acids and their analysis by steady-state fluorescence spectroscopy. Using this method, we investigate the hydration of functionally important regions of dehalogenases. The experimental results are compared to findings from molecular dynamics simulations.
ER  -

AMARO, M., Jan BREZOVSKÝ, Silvia KOVÁČOVÁ, J. SYKORA, David BEDNÁŘ, Václav NĚMEC, Veronika LIŠKOVÁ, Nagendra Prasad KURUMBANG, Koen BEERENS, Radka CHALOUPKOVÁ, Kamil PARUCH, Martina HOF and Jiří DAMBORSKÝ. Site-specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence. \textit{Journal of the American Chemical Society}. 2015, vol.~137, No~15, p.~4988-4992. ISSN~0002-7863. Available from: https://dx.doi.org/10.1021/jacs.5b01681.

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