J 2015

Site-specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence.

AMARO, M., Jan BREZOVSKÝ, Silvia KOVÁČOVÁ, J. SYKORA, David BEDNÁŘ et. al.

Basic information

Original name

Site-specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence.

Authors

AMARO, M. (620 Portugal), Jan BREZOVSKÝ (203 Czech Republic, belonging to the institution), Silvia KOVÁČOVÁ (703 Slovakia, belonging to the institution), J. SYKORA (203 Czech Republic), David BEDNÁŘ (203 Czech Republic, belonging to the institution), Václav NĚMEC (203 Czech Republic, belonging to the institution), Veronika LIŠKOVÁ (203 Czech Republic, belonging to the institution), Nagendra Prasad KURUMBANG (524 Nepal, belonging to the institution), Koen BEERENS (56 Belgium, belonging to the institution), Radka CHALOUPKOVÁ (203 Czech Republic, belonging to the institution), Kamil PARUCH (203 Czech Republic, belonging to the institution), Martina HOF (276 Germany) and Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of the American Chemical Society, 2015, 0002-7863

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 13.038

RIV identification code

RIV/00216224:14310/15:00080814

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1021/jacs.5b01681

UT WoS

000353606700029

Keywords in English

dehalogenases;fluorescence spectroscopy;molecular dynamics simulations

Tags

AKR, rivok
Změněno: 21/3/2017 07:53, prof. Mgr. Jiří Damborský, Dr.

Abstract

V originále

Hydration of proteins profoundly affects their functions. We describe a simple and general method for site-specific analysis of protein hydration based on the in vivo incorporation of fluorescent unnatural amino acids and their analysis by steady-state fluorescence spectroscopy. Using this method, we investigate the hydration of functionally important regions of dehalogenases. The experimental results are compared to findings from molecular dynamics simulations.

Links

EE2.3.30.0037, research and development project
Name: Zaměstnáním nejlepších mladých vědců k rozvoji mezinárodní spolupráce
GAP207/12/0775, research and development project
Name: Strukturně-funkční vztahy haloalkan dehalogenas
Investor: Czech Science Foundation
LM2010005, research and development project
Name: Velká infrastruktura CESNET (Acronym: VI CESNET)
Investor: Ministry of Education, Youth and Sports of the CR
LO1214, research and development project
Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)
Investor: Ministry of Education, Youth and Sports of the CR
Displayed: 5/11/2024 06:38