Site-specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence.

AMARO, M., Jan BREZOVSKÝ, Silvia KOVÁČOVÁ, J. SYKORA, David BEDNÁŘ, Václav NĚMEC, Veronika LIŠKOVÁ, Nagendra Prasad KURUMBANG, Koen BEERENS, Radka CHALOUPKOVÁ, Kamil PARUCH, Martina HOF and Jiří DAMBORSKÝ. Site-specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence. Journal of the American Chemical Society. 2015, vol. 137, No 15, p. 4988-4992. ISSN 0002-7863. Available from: https://dx.doi.org/10.1021/jacs.5b01681.

Basic information

• Original name: Site-specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence.
• Authors: AMARO, M. (620 Portugal), Jan BREZOVSKÝ (203 Czech Republic, belonging to the institution), Silvia KOVÁČOVÁ (703 Slovakia, belonging to the institution), J. SYKORA (203 Czech Republic), David BEDNÁŘ (203 Czech Republic, belonging to the institution), Václav NĚMEC (203 Czech Republic, belonging to the institution), Veronika LIŠKOVÁ (203 Czech Republic, belonging to the institution), Nagendra Prasad KURUMBANG (524 Nepal, belonging to the institution), Koen BEERENS (56 Belgium, belonging to the institution), Radka CHALOUPKOVÁ (203 Czech Republic, belonging to the institution), Kamil PARUCH (203 Czech Republic, belonging to the institution), Martina HOF (276 Germany) and Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Journal of the American Chemical Society, 2015, 0002-7863.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 13.038
• RIV identification code: RIV/00216224:14310/15:00080814
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1021/jacs.5b01681
• UT WoS: 000353606700029
• Keywords in English: dehalogenases;fluorescence spectroscopy;molecular dynamics simulations
• Tags: AKR, rivok

Abstract

Hydration of proteins profoundly affects their functions. We describe a simple and general method for site-specific analysis of protein hydration based on the in vivo incorporation of fluorescent unnatural amino acids and their analysis by steady-state fluorescence spectroscopy. Using this method, we investigate the hydration of functionally important regions of dehalogenases. The experimental results are compared to findings from molecular dynamics simulations.

Links

• EE2.3.30.0037, research and development project: Name: Zaměstnáním nejlepších mladých vědců k rozvoji mezinárodní spolupráce
• GAP207/12/0775, research and development project: Name: Strukturně-funkční vztahy haloalkan dehalogenas

Investor: Czech Science Foundation

• LM2010005, research and development project: Name: Velká infrastruktura CESNET (Acronym: VI CESNET)

Investor: Ministry of Education, Youth and Sports of the CR

• LO1214, research and development project: Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)

Investor: Ministry of Education, Youth and Sports of the CR