Electrochemical and Spectral Behavior of MicroRNA (miR-34a-5p)

D 2015

Electrochemical and Spectral Behavior of MicroRNA (miR-34a-5p)

VEČEŘOVÁ, Aneta, Kristýna HUDCOVÁ, Iveta PILAŘOVÁ, Michal MASAŘÍK, Libuše TRNKOVÁ et. al.

Basic information

Original name

Electrochemical and Spectral Behavior of MicroRNA (miR-34a-5p)

Authors

VEČEŘOVÁ, Aneta (203 Czech Republic, belonging to the institution), Kristýna HUDCOVÁ (203 Czech Republic, belonging to the institution), Iveta PILAŘOVÁ (203 Czech Republic, belonging to the institution), Michal MASAŘÍK (203 Czech Republic, belonging to the institution) and Libuše TRNKOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

1. vyd. Ústí nad Labem, XXXV. Moderní elektrochemické metody, p. 265-268, 4 pp. 2015

Publisher

Srsenová Lenka - Best servis Ústí nad Labem

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10405 Electrochemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

printed version "print"

RIV identification code

RIV/00216224:14310/15:00083021

Organization unit

Faculty of Science

ISBN

978-80-905221-3-8

UT WoS

000358792900059

Keywords (in Czech)

miR-34a-5p; rakovina; miDNA; cyklická voltametrie; voltametrie s lineárně se měnicím potenciálem; spektroskopie cirkulárního dichroismu; rtuťová elektroda

Keywords in English

miR-34a-5p; human cancer; miDNA; cyclic voltammetry; linear sweep voltammetry; circular dichroic spectroscopy; mercury electrode

Abstract

V originále

MicroRNAs are a family of small noncoding RNAs (21-25 nucleotides), negatively regulating gene expresion at the post-transcriptional level. Their functions are critical to normal cellular processes such as differentiation and apoptosis. recent studies demonstrated that deregulated miRNA expression contributes to the malignant phenotype and for this reason miRNAs play a key role as both diagnostic markers and therapeutic targets for many different tumor types (colorectal cancer, breast cancer, lung cancer, prostate cancer, etc.). Great attention is devoted to the study of miR-34a-5p related to head and neck cancer and also prostate cancer. This miRNA is a direct transcriptional target of p53 and its overexpression after p53 activation mediates some of the key tumor suppressive effects of p53 via induction of growth arrest, apoptisis or senescence. Our contribution is focused on the comparison of the structure of miRNA, based on ribose, and its miDNA analogues, based on deoxyribose and containing U or T in this sequence, by using voltammetric methods (cyclic voltammetry - CV or linear sweep voltammetry - LSV). The voltammetric experiment was completed with CD (circular dichroism) spectra.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

LH13053, research and development project

Name: Chemická a elektrochemická analýza purinových derivátů a jejich komplexů s mědí (Acronym: CECAP)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

VEČEŘOVÁ, Aneta, Kristýna HUDCOVÁ, Iveta PILAŘOVÁ, Michal MASAŘÍK and Libuše TRNKOVÁ. Electrochemical and Spectral Behavior of MicroRNA (miR-34a-5p). In Tomáš Navrátil, Miroslav Fojta, Karolína Schwarzová. XXXV. Moderní elektrochemické metody. 1st ed. Ústí nad Labem: Srsenová Lenka - Best servis Ústí nad Labem, 2015, p. 265-268. ISBN 978-80-905221-3-8.

@inproceedings{1300364,
   author = {Večeřová, Aneta and Hudcová, Kristýna and Pilařová, Iveta and Masařík, Michal and Trnková, Libuše},
   address = {Ústí nad Labem},
   booktitle = {XXXV. Moderní elektrochemické metody},
   edition = {1},
   editor = {Tomáš Navrátil, Miroslav Fojta, Karolína Schwarzová},
   keywords = {miR-34a-5p; human cancer; miDNA; cyclic voltammetry; linear sweep voltammetry; circular dichroic spectroscopy; mercury electrode},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Ústí nad Labem},
   isbn = {978-80-905221-3-8},
   note = {Opraven obor publikace},
   pages = {265-268},
   publisher = {Srsenová Lenka - Best servis Ústí nad Labem},
   title = {Electrochemical and Spectral Behavior of MicroRNA (miR-34a-5p)},
   year = {2015}
}

TY  - JOUR
ID  - 1300364
AU  - Večeřová, Aneta - Hudcová, Kristýna - Pilařová, Iveta - Masařík, Michal - Trnková, Libuše
PY  - 2015
TI  - Electrochemical and Spectral Behavior of MicroRNA (miR-34a-5p)
PB  - Srsenová Lenka - Best servis Ústí nad Labem
CY  - Ústí nad Labem
SN  - 9788090522138
N1  - Opraven obor publikace
KW  - miR-34a-5p
KW  - human cancer
KW  - miDNA
KW  - cyclic voltammetry
KW  - linear sweep voltammetry
KW  - circular dichroic spectroscopy
KW  - mercury electrode
N2  - MicroRNAs are a family of small noncoding RNAs (21-25 nucleotides), negatively regulating gene expresion at the post-transcriptional level. Their functions are critical to normal cellular processes such as differentiation and apoptosis. recent studies demonstrated that deregulated miRNA expression contributes to the malignant phenotype and for this reason miRNAs play a key role as both diagnostic markers and therapeutic targets for many different tumor types (colorectal cancer, breast cancer, lung cancer, prostate cancer, etc.). Great attention is devoted to the study of miR-34a-5p related to head and neck cancer and also prostate cancer. This miRNA is a direct transcriptional target of p53 and its overexpression after p53 activation mediates some of the key tumor suppressive effects of p53 via induction of growth arrest, apoptisis or senescence. Our contribution is focused on the comparison of the structure of miRNA, based on ribose, and its miDNA analogues, based on deoxyribose and containing U or T in this sequence, by using voltammetric methods (cyclic voltammetry - CV or linear sweep voltammetry - LSV). The voltammetric experiment was completed with CD (circular dichroism) spectra.
ER  -

VEČEŘOVÁ, Aneta, Kristýna HUDCOVÁ, Iveta PILAŘOVÁ, Michal MASAŘÍK and Libuše TRNKOVÁ. Electrochemical and Spectral Behavior of MicroRNA (miR-34a-5p). In Tomáš Navrátil, Miroslav Fojta, Karolína Schwarzová. \textit{XXXV. Moderní elektrochemické metody}. 1st ed. Ústí nad Labem: Srsenová Lenka - Best servis Ústí nad Labem, 2015, p.~265-268. ISBN~978-80-905221-3-8.

Detailed Information on Publication Record