Characterization of the Role of Collagen Network Structure and
Composition in Cancer Cell Migration

D 2015

Characterization of the Role of Collagen Network Structure and Composition in Cancer Cell Migration

ANGUIANO, María, Carlos CASTILLA, Martin MAŠKA, Cristina EDERRA, Javier FERNÁNDEZ-MARQUÉS et. al.

Basic information

Original name

Characterization of the Role of Collagen Network Structure and Composition in Cancer Cell Migration

Authors

ANGUIANO, María (724 Spain), Carlos CASTILLA (724 Spain), Martin MAŠKA (203 Czech Republic, guarantor, belonging to the institution), Cristina EDERRA (724 Spain), Javier FERNÁNDEZ-MARQUÉS (724 Spain), Rafael PELÁEZ (724 Spain), Ana ROUZAUT (724 Spain), Arrate MUÑOZ-BARRUTIA (724 Spain), Michal KOZUBEK (203 Czech Republic, belonging to the institution) and Carlos ORTIZ-DE-SOLÓRZANO (724 Spain)

Edition

Milano, 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, p. 8139-8142, 4 pp. 2015

Publisher

IEEE

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10201 Computer sciences, information science, bioinformatics

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

electronic version available online

References:

URL

RIV identification code

RIV/00216224:14330/15:00080823

Organization unit

Faculty of Informatics

ISBN

978-1-4244-9271-8

ISSN

DOI

http://dx.doi.org/10.1109/EMBC.2015.7320283

UT WoS

000371717208103

Keywords in English

collagen network geometry; confocal reflection microscopy; Matrigel; steerable filtering; pore size; cell migration

Abstract

V originále

The geometry of 3D collagen networks is a key factor that influences the behavior of live cells within extracellular matrices. This paper presents a method for automatic quantification of the 3D collagen network geometry with fiber resolution in confocal reflection microscopy images. The proposed method is based on a smoothing filter and binarization of the collagen network followed by a fiber reconstruction algorithm. The method is validated on 3D collagen gels with various collagen and Matrigel concentrations. The results reveal that Matrigel affects the collagen network geometry by decreasing the network pore size while preserving the fiber length and fiber persistence length. The influence of network composition and geometry, especially pore size, is preliminarily analyzed by quantifying the migration patterns of lung cancer cells within microfluidic devices filled with three different hydrogel types. The experiments reveal that Matrigel, while decreasing pore size, stimulates cell migration. Further studies on this relationship could be instrumental for the study of cancer metastasis and other biological processes involving cell migration.

Links

EE2.3.30.0009, research and development project

Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GBP302/12/G157, research and development project

Name: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Czech Science Foundation

Citovat

ANGUIANO, María, Carlos CASTILLA, Martin MAŠKA, Cristina EDERRA, Javier FERNÁNDEZ-MARQUÉS, Rafael PELÁEZ, Ana ROUZAUT, Arrate MUÑOZ-BARRUTIA, Michal KOZUBEK and Carlos ORTIZ-DE-SOLÓRZANO. Characterization of the Role of Collagen Network Structure and Composition in Cancer Cell Migration. Online. In 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Milano: IEEE, 2015, p. 8139-8142. ISBN 978-1-4244-9271-8. Available from: https://dx.doi.org/10.1109/EMBC.2015.7320283.

@inproceedings{1300413,
   author = {Anguiano, María and Castilla, Carlos and Maška, Martin and Ederra, Cristina and FernándezandMarqués, Javier and Peláez, Rafael and Rouzaut, Ana and MuñozandBarrutia, Arrate and Kozubek, Michal and OrtizanddeandSolórzano, Carlos},
   address = {Milano},
   booktitle = {37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society},
   doi = {http://dx.doi.org/10.1109/EMBC.2015.7320283},
   keywords = {collagen network geometry; confocal reflection microscopy; Matrigel; steerable filtering; pore size; cell migration},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {Milano},
   isbn = {978-1-4244-9271-8},
   pages = {8139-8142},
   publisher = {IEEE},
   title = {Characterization of the Role of Collagen Network Structure and Composition in Cancer Cell Migration},
   url = {http://dx.doi.org/10.1109/EMBC.2015.7320283},
   year = {2015}
}

TY  - JOUR
ID  - 1300413
AU  - Anguiano, María - Castilla, Carlos - Maška, Martin - Ederra, Cristina - Fernández-Marqués, Javier - Peláez, Rafael - Rouzaut, Ana - Muñoz-Barrutia, Arrate - Kozubek, Michal - Ortiz-de-Solórzano, Carlos
PY  - 2015
TI  - Characterization of the Role of Collagen Network Structure and Composition in Cancer Cell Migration
PB  - IEEE
CY  - Milano
SN  - 9781424492718
KW  - collagen network geometry
KW  - confocal reflection microscopy
KW  - Matrigel
KW  - steerable filtering
KW  - pore size
KW  - cell migration
UR  - http://dx.doi.org/10.1109/EMBC.2015.7320283
N2  - The geometry of 3D collagen networks is a key factor that influences the behavior of live cells within extracellular matrices. This paper presents a method for automatic quantification of the 3D collagen network geometry with fiber resolution in confocal reflection microscopy images. The proposed method is based on a smoothing filter and binarization of the collagen network followed by a fiber reconstruction algorithm. The method is validated on 3D collagen gels with various collagen and Matrigel concentrations. The results reveal that Matrigel affects the collagen network geometry by decreasing the network pore size while preserving the fiber length and fiber persistence length. The influence of network composition and geometry, especially pore size, is preliminarily analyzed by quantifying the migration patterns of lung cancer cells within microfluidic devices filled with three different hydrogel types. The experiments reveal that Matrigel, while decreasing pore size, stimulates cell migration. Further studies on this relationship could be instrumental for the study of cancer metastasis and other biological processes involving cell migration.
ER  -

ANGUIANO, María, Carlos CASTILLA, Martin MAŠKA, Cristina EDERRA, Javier FERNÁNDEZ-MARQUÉS, Rafael PELÁEZ, Ana ROUZAUT, Arrate MUÑOZ-BARRUTIA, Michal KOZUBEK and Carlos ORTIZ-DE-SOLÓRZANO. Characterization of the Role of Collagen Network Structure and Composition in Cancer Cell Migration. Online. In \textit{37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society}. Milano: IEEE, 2015, p.~8139-8142. ISBN~978-1-4244-9271-8. Available from: https://dx.doi.org/10.1109/EMBC.2015.7320283.

Detailed Information on Publication Record