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@inproceedings{1300413,
author = {Anguiano, María and Castilla, Carlos and Maška, Martin and Ederra, Cristina and FernándezandMarqués, Javier and Peláez, Rafael and Rouzaut, Ana and MuñozandBarrutia, Arrate and Kozubek, Michal and OrtizanddeandSolórzano, Carlos},
address = {Milano},
booktitle = {37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society},
doi = {http://dx.doi.org/10.1109/EMBC.2015.7320283},
keywords = {collagen network geometry; confocal reflection microscopy; Matrigel; steerable filtering; pore size; cell migration},
howpublished = {elektronická verze "online"},
language = {eng},
location = {Milano},
isbn = {978-1-4244-9271-8},
pages = {8139-8142},
publisher = {IEEE},
title = {Characterization of the Role of Collagen Network Structure and Composition in Cancer Cell Migration},
url = {http://dx.doi.org/10.1109/EMBC.2015.7320283},
year = {2015}
}
TY - JOUR
ID - 1300413
AU - Anguiano, María - Castilla, Carlos - Maška, Martin - Ederra, Cristina - Fernández-Marqués, Javier - Peláez, Rafael - Rouzaut, Ana - Muñoz-Barrutia, Arrate - Kozubek, Michal - Ortiz-de-Solórzano, Carlos
PY - 2015
TI - Characterization of the Role of Collagen Network Structure and Composition in Cancer Cell Migration
PB - IEEE
CY - Milano
SN - 9781424492718
KW - collagen network geometry
KW - confocal reflection microscopy
KW - Matrigel
KW - steerable filtering
KW - pore size
KW - cell migration
UR - http://dx.doi.org/10.1109/EMBC.2015.7320283
N2 - The geometry of 3D collagen networks is a key factor that influences the behavior of live cells within extracellular matrices. This paper presents a method for automatic quantification of the 3D collagen network geometry with fiber resolution in confocal reflection microscopy images. The proposed method is based on a smoothing filter and binarization of the collagen network followed by a fiber reconstruction algorithm. The method is validated on 3D collagen gels with various collagen and Matrigel concentrations. The results reveal that Matrigel affects the collagen network geometry by decreasing the network pore size while preserving the fiber length and fiber persistence length. The influence of network composition and geometry, especially pore size, is preliminarily analyzed by quantifying the migration patterns of lung cancer cells within microfluidic devices filled with three different hydrogel types. The experiments reveal that Matrigel, while decreasing pore size, stimulates cell migration. Further studies on this relationship could be instrumental for the study of cancer metastasis and other biological processes involving cell migration.
ER -
ANGUIANO, María, Carlos CASTILLA, Martin MAŠKA, Cristina EDERRA, Javier FERNÁNDEZ-MARQUÉS, Rafael PELÁEZ, Ana ROUZAUT, Arrate MUÑOZ-BARRUTIA, Michal KOZUBEK and Carlos ORTIZ-DE-SOLÓRZANO. Characterization of the Role of Collagen Network Structure and Composition in Cancer Cell Migration. In \textit{37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society}. Milano: IEEE. p.~8139-8142. ISBN~978-1-4244-9271-8. doi:10.1109/EMBC.2015.7320283. 2015.
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