This paper examines the transformation of the post-industrial city in terms of its temporal structure. It takes concepts of time geography, routine and rhythmicity of the classic Lund school, Lefebvre’s analysis of rhythms and Crang’s geographic application of the chronotope concept as its starting points. Analysing changes in the city bus transport services in Brno between 1989 and 2009, the paper attempts to capture in empirical terms the onset of the post-industrial phase of the city’s development. While temporality of an industrial city can be characterized by a shared rhythm determined by a small number of dominant pacemakers (industrial plants), the deindustrialized city is associated with a significant weakening of such pacemakers cutting across the society and thus with a distinctive individualization of urban rhythmicity.