RUDÁ, Jana, Petra AMCHOVÁ, Zuzana BABINSKÁ, Ladislav DUŠEK, Vincenzo MICALE and Alexandra ŠULCOVÁ. Sex differences in the reinstatement of methamphetamine seeking after forced abstinence in Sprague-Dawley rats. Frontiers in Psychiatry. Lausanne: Frontiers, 2015, vol. 6, No 91, p. 1-8. ISSN 1664-0640. doi:10.3389/fpsyt.2015.00091.
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Basic information
Original name Sex differences in the reinstatement of methamphetamine seeking after forced abstinence in Sprague-Dawley rats
Name in Czech Mezipohlavní rozdíly ve vyhledávání metamfetaminu po nucené abstinenci u Sprague-Dawley potkana
Authors RUDÁ, Jana (203 Czech Republic, guarantor, belonging to the institution), Petra AMCHOVÁ (203 Czech Republic, belonging to the institution), Zuzana BABINSKÁ (703 Slovakia, belonging to the institution), Ladislav DUŠEK (203 Czech Republic, belonging to the institution), Vincenzo MICALE (380 Italy, belonging to the institution) and Alexandra ŠULCOVÁ (203 Czech Republic, belonging to the institution).
Edition Frontiers in Psychiatry, Lausanne, Frontiers, 2015, 1664-0640.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30104 Pharmacology and pharmacy
Country of publisher Switzerland
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14110/15:00083330
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.3389/fpsyt.2015.00091
UT WoS 000364467900001
Keywords in English methamphetamine; reinstatement of drug-seeking behavior; forced abstinence; sex/gender differences; Sprague-Dawley rats
Tags EL OK, podil
Tags International impact, Reviewed
Changed by Changed by: Ing. Mgr. Věra Pospíšilíková, učo 9005. Changed: 16. 12. 2015 12:45.
Abstract
Preventing relapse to drug abuse is one of the struggles faced by clinicians in order to treat patients with substance use disorders (DSM-5). There is a large body of clinical evidence suggesting differential characteristics of the disorder in men and women, which is in line with preclinical findings as well. The aim of this study was to assess differences in relapse-like behavior in methamphetamine (METH) seeking after a period of forced abstinence, which simulates the real clinical situation very well. Findings from such study might add new insights in gender differences in relapse mechanisms to previous studies, which employ a classical drug or cue-induced reinstatement procedure following the extinction training. Adult male and female Sprague-Dawley rats were used in IV self-administration procedure conducted in operant boxes using nose-poke operandi (Coulborn Instruments, USA). Active nose-poke resulted in activation of the infusion pump to deliver one intravenous infusion of METH (0.08 mg/kg). After baseline drug intake was established (maintenance phase), a period of forced abstinence was initiated and rats were kept singly in their home cages for 14 days. Finally, one reinstatement session in operant boxes was conducted. Females were found to self-administer significantly lower dose of METH. The relapse rate was assessed as a number of active nose-pokes during the reinstatement session, expressed as a percentage of active nose-poking during the maintenance phase. Females displayed approximately 300% of active nose-pokes compared to 50% in males. This indicates higher vulnerability to relapse of METH seeking behavior in female rats. This effect was detected in all females, independently of current phase of their estrous cycle. Therefore, this paradigm using operant drug self-administration and reinstatement of drug-seeking after forced abstinence model can be used for preclinical screening for potential new anti-relapse medications specific for women.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
MUNI/A/1116/2014, interní kód MUName: Nové farmakologické mechanizmy v preklinickém i klinickém vývoji léčiv
Investor: Masaryk University, Category A
MUNI/11/InGA09/2012, interní kód MUName: Vliv farmakologického ovlivnění serotonergního systému na metamfetaminovou závislost v animálním modelu komorbidity deprese a závislosti (Acronym: Serotonin, deprese a závislost)
Investor: Masaryk University
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