J 2015

An improved method for the visualization of conductive vessels in Arabidopsis thaliana inflorescence stems

JUPA, Radek, Vojtěch DIDI, Jan HEJÁTKO and Vít GLOSER

Basic information

Original name

An improved method for the visualization of conductive vessels in Arabidopsis thaliana inflorescence stems

Authors

JUPA, Radek (203 Czech Republic, belonging to the institution), Vojtěch DIDI (203 Czech Republic, belonging to the institution), Jan HEJÁTKO (203 Czech Republic, belonging to the institution) and Vít GLOSER (203 Czech Republic, guarantor, belonging to the institution)

Edition

Frontiers in Plant Science, Frontiers Research Foundation, 2015, 1664-462X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 4.495

RIV identification code

RIV/00216224:14310/15:00083331

Organization unit

Faculty of Science

UT WoS

000352616400001

Keywords in English

conductive elements; dye perfusion; fluorescence; Fluorescent Brightener 28; hydraulic conductivity; vessel; xylem

Tags

Tags

International impact, Reviewed
Změněno: 22/11/2017 14:31, RNDr. Radek Jupa, Ph.D.

Abstract

V originále

Dye perfusion is commonly used for the identification of conductive elements important for the study of xylem development as well as precise hydraulic estimations. The tiny size of inflorescence stems, the small amount of vessels in close arrangement, and high hydraulic resistivity delimit the use of the method for quantification of the water conductivity of Arabidopsis thaliana, one of the recently most extensively used plant models. Here, we present an extensive adjustment to the method in order to reliably identify individual functional (conductive) vessels.