| NEJDL, Lukas, Hoai Viet NGUYEN, Lukáš RICHTERA, Soňa KŘÍŽKOVÁ, Roman GURÁŇ, Michal MASAŘÍK, David HYNEK, Zbynek HEGER, Karin LUNDBERG, Kristofer ERIKSON, Vojtěch ADAM and René KIZEK. Label-free bead-based metallothionein electrochemical immunosensor. Electrophoresis. Hoboken: Wiley-Blackwell, 2015, vol. 36, No 16, p. 1894-1904. ISSN 0173-0835. Available from: https://dx.doi.org/10.1002/elps.201500069. |
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@article{1305859,
author = {Nejdl, Lukas and Nguyen, Hoai Viet and Richtera, Lukáš and Křížková, Soňa and Guráň, Roman and Masařík, Michal and Hynek, David and Heger, Zbynek and Lundberg, Karin and Erikson, Kristofer and Adam, Vojtěch and Kizek, René},
article_location = {Hoboken},
article_number = {16},
doi = {http://dx.doi.org/10.1002/elps.201500069},
keywords = {Immunosensor; Label-free; Metallothionein; Superparamagnetic particles},
language = {eng},
issn = {0173-0835},
journal = {Electrophoresis},
title = {Label-free bead-based metallothionein electrochemical immunosensor},
url = {http://dx.doi.org/10.1002/elps.201500069},
volume = {36},
year = {2015}
}
TY - JOUR
ID - 1305859
AU - Nejdl, Lukas - Nguyen, Hoai Viet - Richtera, Lukáš - Křížková, Soňa - Guráň, Roman - Masařík, Michal - Hynek, David - Heger, Zbynek - Lundberg, Karin - Erikson, Kristofer - Adam, Vojtěch - Kizek, René
PY - 2015
TI - Label-free bead-based metallothionein electrochemical immunosensor
JF - Electrophoresis
VL - 36
IS - 16
SP - 1894-1904
EP - 1894-1904
PB - Wiley-Blackwell
SN - 01730835
KW - Immunosensor
KW - Label-free
KW - Metallothionein
KW - Superparamagnetic particles
UR - http://dx.doi.org/10.1002/elps.201500069
N2 - A novel microfluidic label-free bead-based metallothionein immunosensors was designed. To the surface of superparamagnetic agarose beads coated with protein A, polyclonal chicken IgY specifically recognizing metallothionein (MT) were immobilized via rabbit IgG. The Brdicka reaction was used for metallothionein detection in a microfluidic printed 3D chip. Assembled chip consisted of a single copper wire coated with a thin layer of amalgam as working electrode. Optimization of MT detection using designed microfluidic chip was performed in stationary system as well as in the flow arrangement at various flow rates (0-1800 mikroL/min). In stationary arrangement it is possible to detect MT concentrations up to 30 ng/mL level, flow arrangement allows reliable detection of even lower concentration (12.5 ng/mL). The assembled miniature flow chip was subsequently tested for the detection of MT elevated levels (at approx. level 100 mikrog/mL) in real samples of patients with cancer. The stability of constructed device for metallothionein detection in flow arrangement was found to be several days without any maintenance needed.
ER -
NEJDL, Lukas, Hoai Viet NGUYEN, Lukáš RICHTERA, Soňa KŘÍŽKOVÁ, Roman GURÁŇ, Michal MASAŘÍK, David HYNEK, Zbynek HEGER, Karin LUNDBERG, Kristofer ERIKSON, Vojtěch ADAM and René KIZEK. Label-free bead-based metallothionein electrochemical immunosensor. \textit{Electrophoresis}. Hoboken: Wiley-Blackwell, 2015, vol.~36, No~16, p.~1894-1904. ISSN~0173-0835. Available from: https://dx.doi.org/10.1002/elps.201500069.
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