Detailed Information on Publication Record
2015
Diffusion Kurtosis Imaging Detects Microstructural Alterations in Brain of alpha-Synuclein Overexpressing Transgenic Mouse Model of Parkinson’s Disease: A Pilot Study
KHAIRNAR, Amit Suresh, Peter LATTA, Eva DRAŽANOVÁ, Jana RUDÁ, Nikoletta SZABÓ et. al.Basic information
Original name
Diffusion Kurtosis Imaging Detects Microstructural Alterations in Brain of alpha-Synuclein Overexpressing Transgenic Mouse Model of Parkinson’s Disease: A Pilot Study
Name in Czech
Diffusion Kurtosis Imaging detekuje mikrostrukturální změny v mozku myší alfa-synukleinového transgenního modelu Parkinsonovy choroby: pilotní studie
Authors
KHAIRNAR, Amit Suresh (356 India, belonging to the institution), Peter LATTA (703 Slovakia, belonging to the institution), Eva DRAŽANOVÁ (203 Czech Republic, belonging to the institution), Jana RUDÁ (203 Czech Republic, belonging to the institution), Nikoletta SZABÓ (348 Hungary), Anas ARAB (760 Syrian Arab Republic, belonging to the institution), Birgit HUTTER-PAIER (40 Austria), Daniel HAVAS (40 Austria), Manfred WINDISCH (40 Austria), Alexandra ŠULCOVÁ (203 Czech Republic, belonging to the institution), Zenon STARČUK (203 Czech Republic, belonging to the institution) and Irena REKTOROVÁ (203 Czech Republic, guarantor, belonging to the institution)
Edition
Neurotoxicity research, New York, Springer, 2015, 1029-8428
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30000 3. Medical and Health Sciences
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 3.140
RIV identification code
RIV/00216224:14740/15:00083366
Organization unit
Central European Institute of Technology
UT WoS
000362028700001
Keywords in English
Diffusion kurtosis imaging; a-Synuclein; TNWT-61; Parkinson’s disease; Transgenic mice; TBSS
Tags
International impact, Reviewed
Změněno: 29/4/2016 13:07, Mgr. Eva Špillingová
Abstract
V originále
Evidence suggests that accumulation and aggregation of alpha-synuclein contribute to the pathogenesis of Parkinson's disease (PD). The aim of this study was to evaluate whether diffusion kurtosis imaging (DKI) will provide a sensitive tool for differentiating between alpha-synuclein-overexpressing transgenic mouse model of PD (TNWT-61) and wild-type (WT) littermates. This experiment was designed as a proof-of-concept study and forms a part of a complex protocol and ongoing translational research. Nine-month-old TNWT-61 mice and age-matched WT littermates underwent behavioral tests to monitor motor impairment and MRI scanning using 9.4 Tesla system in vivo. Tract-based spatial statistics (TBSS) and the DKI protocol were used to compare the whole brain white matter of TNWT-61 and WT mice. In addition, region of interest (ROI) analysis was performed in gray matter regions such as substantia nigra, striatum, hippocampus, sensorimotor cortex, and thalamus known to show higher accumulation of alpha-synuclein. For the ROI analysis, both DKI (6 b-values) protocol and conventional (2 b-values) diffusion tensor imaging (cDTI) protocol were used. TNWT-61 mice showed significant impairment of motor coordination. With the DKI protocol, mean, axial, and radial kurtosis were found to be significantly elevated, whereas mean and radial diffusivity were decreased in the TNWT-61 group compared to that in the WT controls with both TBSS and ROI analysis. With the cDTI protocol, the ROI analysis showed decrease in all diffusivity parameters in TNWT-61 mice. The current study provides evidence that DKI by providing both kurtosis and diffusivity parameters gives unique information that is complementary to cDTI for in vivo detection of pathological changes that underlie PD-like symptomatology in TNWT-61 mouse model of PD. This result is a crucial step in search for a candidate diagnostic biomarker with translational potential and relevance for human studies.
Links
ED1.1.00/02.0068, research and development project |
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EE2.3.30.0009, research and development project |
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