Detailed Information on Publication Record
2015
Plant alcaloid and probiotics increase resistance of honeybees to nematobacterial infection
HYRŠL, Pavel, Pavel DOBEŠ, Libor VOJTEK, Jakub BERKA, Jana HURYCHOVÁ et. al.Basic information
Original name
Plant alcaloid and probiotics increase resistance of honeybees to nematobacterial infection
Name in Czech
Plant alcaloid and probiotics increase resistance of honeybees to nematobacterial infection
Authors
HYRŠL, Pavel (203 Czech Republic, guarantor, belonging to the institution), Pavel DOBEŠ (203 Czech Republic, belonging to the institution), Libor VOJTEK (203 Czech Republic, belonging to the institution), Jakub BERKA (203 Czech Republic, belonging to the institution), Jana HURYCHOVÁ (203 Czech Republic, belonging to the institution), Jaroslav HAVLIK (203 Czech Republic), Martin KAMLER (203 Czech Republic), Zuzana HRONCOVÁ (203 Czech Republic), Jiří KILLER (203 Czech Republic), Jan TYL (203 Czech Republic) and Dalibor TITĚRA (203 Czech Republic)
Edition
15th Meeting of the IOBCWPRS Working Group "Microbial and Nematode Control of Invertebrathe Pests" 2015
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
30102 Immunology
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/15:00080479
Organization unit
Faculty of Science
Keywords (in Czech)
entomopatogenní hlístice; včely; imunita; probiotika
Keywords in English
entomopathogenic nematodes; honeybees; immunity; probiotics
Tags
International impact, Reviewed
Změněno: 10/2/2016 15:25, doc. RNDr. Pavel Hyršl, Ph.D.
V originále
Entomopathogenic nematodes (EPNs) are obligate insect parasites that are symbiotically associated with specific entomopathogenic bacteria. These nematobacterial complexes are highly pathogenic to insect hosts and therefore used in biological control of insect pests. As was shown previously, EPNs can be used also as a natural infection model and a powerful tool to study insect immunity. In this study we show that also honeybee larvae are suitable hosts for nematobacterial complex that offer an excellent environment for successful development of both nematodes and their symbiotic bacteria. Here we used EPNs for evaluation of the overall immune resistance of honeybee larvae treated with potentially immuno-modulating substances (plant alcaloid sanguinarin and probiotics). Honeybee larvae were infected with 10 IJ of Heterorhabditis bacteriophora or Steinernema feltiae per larva, both carrying their symbiotic bacteria, and then scored for mortality caused by the nematobacterial infection. In comparison to untreated honeybee larvae we observed the decrease in mortality of approx. 35 % in case of H. bacteriophora and approx. 10% upon S. feltiae infection in larvae treated by selected substances. Both sanguinarin and probiotic treatment showed similar protective effect that was less significant in highly virulent S. feltiae. This is the first record that the nematobacterial infection was used for evaluation of immune status of beneficial insect. We suggest and propose this method as a valuable tool for immunity testing in honeybees as well as in other insects. Our research is supported by grant from the Ministry of Agriculture of Czech Republic (project No. QJ1210047) and by the program CZ.1.07/2.3.00/30.0009 co-financed from European Social Fund and the state budget of the Czech Republic.
In Czech
Entomopathogenic nematodes (EPNs) are obligate insect parasites that are symbiotically associated with specific entomopathogenic bacteria. These nematobacterial complexes are highly pathogenic to insect hosts and therefore used in biological control of insect pests. As was shown previously, EPNs can be used also as a natural infection model and a powerful tool to study insect immunity. In this study we show that also honeybee larvae are suitable hosts for nematobacterial complex that offer an excellent environment for successful development of both nematodes and their symbiotic bacteria. Here we used EPNs for evaluation of the overall immune resistance of honeybee larvae treated with potentially immuno-modulating substances (plant alcaloid sanguinarin and probiotics). Honeybee larvae were infected with 10 IJ of Heterorhabditis bacteriophora or Steinernema feltiae per larva, both carrying their symbiotic bacteria, and then scored for mortality caused by the nematobacterial infection. In comparison to untreated honeybee larvae we observed the decrease in mortality of approx. 35 % in case of H. bacteriophora and approx. 10% upon S. feltiae infection in larvae treated by selected substances. Both sanguinarin and probiotic treatment showed similar protective effect that was less significant in highly virulent S. feltiae. This is the first record that the nematobacterial infection was used for evaluation of immune status of beneficial insect. We suggest and propose this method as a valuable tool for immunity testing in honeybees as well as in other insects. Our research is supported by grant from the Ministry of Agriculture of Czech Republic (project No. QJ1210047) and by the program CZ.1.07/2.3.00/30.0009 co-financed from European Social Fund and the state budget of the Czech Republic.
Links
| CZ.1.07/2.3.00/30.0009, interní kód MU (CEP code: EE2.3.30.0009) |
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| QJ1210047, research and development project |
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