HODÁKOVÁ, Júlia, Jan PREISLER, František FORET and Petr KUBÁŇ. Sensitive determination of glutathione in biological samples by capillary electrophoresis with green (515 nm) laser-induced fluorescence detection. Journal of Chromatography A. AMSTERDAM: Elsevier, 2015, vol. 1391, April, p. 102-108. ISSN 0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2015.02.062.
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Basic information
Original name Sensitive determination of glutathione in biological samples by capillary electrophoresis with green (515 nm) laser-induced fluorescence detection
Authors HODÁKOVÁ, Júlia (703 Slovakia, belonging to the institution), Jan PREISLER (203 Czech Republic, belonging to the institution), František FORET (203 Czech Republic, belonging to the institution) and Petr KUBÁŇ (203 Czech Republic, belonging to the institution).
Edition Journal of Chromatography A, AMSTERDAM, Elsevier, 2015, 0021-9673.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10406 Analytical chemistry
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 3.926
RIV identification code RIV/00216224:14310/15:00080903
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1016/j.chroma.2015.02.062
UT WoS 000353427000012
Keywords in English Capillary electrophoresis; Laser-induced fluorescence; Glutathione; Eosin-5-maleimide
Tags AKR, rivok
Tags International impact, Reviewed
Changed by Changed by: prof. Mgr. Jan Preisler, Ph.D., učo 45329. Changed: 15/2/2018 22:06.
Abstract
A new sensitive capillary electrophoretic method with laser-induced fluorescence (LIF) was developed for quantitation of glutathione (GSH) in biological samples. Eosin-5-maleimide was used to label the GSH molecule and the formed conjugate was separated in a 15 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid electrolyte at pH 7.0 in less than 3 min. The conjugate was detected with an in-house built LIF system, utilizing an inexpensive 515 nm diode laser module. Studies were performed to optimize the derivatizatiory(the ratio of reagent to analyte, the reaction time, pH, etc.) and separation conditions. Sensitive detection of GSH at concentrations as low as 0.18 nM was obtained. The method was applied in the analysis of biological fluids (exhaled breath condensate, saliva) and was found to be suitable for determination of GSH in these samples at trace levels below 1 nM. To the best of our knowledge, this is the first report on determination of GSH in exhaled breath condensate by capillary electrophoresis (CE). (C) 2015 Elsevier B.V. All rights reserved.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
GA13-21919S, research and development projectName: Vývoj neinvazivních metod odběru vzorků a analýzy v point-of-care dignostice biologických materiálů.
Investor: Czech Science Foundation
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