Sensitive determination of glutathione in biological samples by
capillary electrophoresis with green (515 nm) laser-induced
fluorescence detection

J 2015

Sensitive determination of glutathione in biological samples by capillary electrophoresis with green (515 nm) laser-induced fluorescence detection

HODÁKOVÁ, Júlia, Jan PREISLER, František FORET and Petr KUBÁŇ

Basic information

Original name

Sensitive determination of glutathione in biological samples by capillary electrophoresis with green (515 nm) laser-induced fluorescence detection

Authors

HODÁKOVÁ, Júlia (703 Slovakia, belonging to the institution), Jan PREISLER (203 Czech Republic, belonging to the institution), František FORET (203 Czech Republic, belonging to the institution) and Petr KUBÁŇ (203 Czech Republic, belonging to the institution)

Edition

Journal of Chromatography A, AMSTERDAM, Elsevier, 2015, 0021-9673

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.926

RIV identification code

RIV/00216224:14310/15:00080903

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.chroma.2015.02.062

UT WoS

000353427000012

Keywords in English

Capillary electrophoresis; Laser-induced fluorescence; Glutathione; Eosin-5-maleimide

Abstract

V originále

A new sensitive capillary electrophoretic method with laser-induced fluorescence (LIF) was developed for quantitation of glutathione (GSH) in biological samples. Eosin-5-maleimide was used to label the GSH molecule and the formed conjugate was separated in a 15 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid electrolyte at pH 7.0 in less than 3 min. The conjugate was detected with an in-house built LIF system, utilizing an inexpensive 515 nm diode laser module. Studies were performed to optimize the derivatizatiory(the ratio of reagent to analyte, the reaction time, pH, etc.) and separation conditions. Sensitive detection of GSH at concentrations as low as 0.18 nM was obtained. The method was applied in the analysis of biological fluids (exhaled breath condensate, saliva) and was found to be suitable for determination of GSH in these samples at trace levels below 1 nM. To the best of our knowledge, this is the first report on determination of GSH in exhaled breath condensate by capillary electrophoresis (CE). (C) 2015 Elsevier B.V. All rights reserved.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

GA13-21919S, research and development project

Name: Vývoj neinvazivních metod odběru vzorků a analýzy v point-of-care dignostice biologických materiálů.

Investor: Czech Science Foundation

Citovat

HODÁKOVÁ, Júlia, Jan PREISLER, František FORET and Petr KUBÁŇ. Sensitive determination of glutathione in biological samples by capillary electrophoresis with green (515 nm) laser-induced fluorescence detection. Journal of Chromatography A. AMSTERDAM: Elsevier, 2015, vol. 1391, April, p. 102-108. ISSN 0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2015.02.062.

@article{1306292,
   author = {Hodáková, Júlia and Preisler, Jan and Foret, František and Kubáň, Petr},
   article_location = {AMSTERDAM},
   article_number = {April},
   doi = {http://dx.doi.org/10.1016/j.chroma.2015.02.062},
   keywords = {Capillary electrophoresis; Laser-induced fluorescence; Glutathione; Eosin-5-maleimide},
   language = {eng},
   issn = {0021-9673},
   journal = {Journal of Chromatography A},
   title = {Sensitive determination of glutathione in biological samples by capillary electrophoresis with green (515 nm) laser-induced fluorescence detection},
   volume = {1391},
   year = {2015}
}

TY  - JOUR
ID  - 1306292
AU  - Hodáková, Júlia - Preisler, Jan - Foret, František - Kubáň, Petr
PY  - 2015
TI  - Sensitive determination of glutathione in biological samples by capillary electrophoresis with green (515 nm) laser-induced fluorescence detection
JF  - Journal of Chromatography A
VL  - 1391
IS  - April
SP  - 102-108
EP  - 102-108
PB  - Elsevier
SN  - 00219673
KW  - Capillary electrophoresis
KW  - Laser-induced fluorescence
KW  - Glutathione
KW  - Eosin-5-maleimide
N2  - A new sensitive capillary electrophoretic method with laser-induced fluorescence (LIF) was developed for quantitation of glutathione (GSH) in biological samples. Eosin-5-maleimide was used to label the GSH molecule and the formed conjugate was separated in a 15 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid electrolyte at pH 7.0 in less than 3 min. The conjugate was detected with an in-house built LIF system, utilizing an inexpensive 515 nm diode laser module. Studies were performed to optimize the derivatizatiory(the ratio of reagent to analyte, the reaction time, pH, etc.) and separation conditions. Sensitive detection of GSH at concentrations as low as 0.18 nM was obtained. The method was applied in the analysis of biological fluids (exhaled breath condensate, saliva) and was found to be suitable for determination of GSH in these samples at trace levels below 1 nM. To the best of our knowledge, this is the first report on determination of GSH in exhaled breath condensate by capillary electrophoresis (CE). (C) 2015 Elsevier B.V. All rights reserved.
ER  -

HODÁKOVÁ, Júlia, Jan PREISLER, František FORET and Petr KUBÁŇ. Sensitive determination of glutathione in biological samples by capillary electrophoresis with green (515 nm) laser-induced fluorescence detection. \textit{Journal of Chromatography A}. AMSTERDAM: Elsevier, 2015, vol.~1391, April, p.~102-108. ISSN~0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2015.02.062.

Detailed Information on Publication Record