SENSITIVE DETERMINATION OF GLUTATHIONE IN VARIOUS BIOLOGICAL
SAMPLES BY CAPILLARY ELECTROPHORESIS WITH GREEN LASER INDUCED
FLUORESCENCE DETECTION

D 2014

SENSITIVE DETERMINATION OF GLUTATHIONE IN VARIOUS BIOLOGICAL SAMPLES BY CAPILLARY ELECTROPHORESIS WITH GREEN LASER INDUCED FLUORESCENCE DETECTION

LAČNÁ, Júlia, Jan PREISLER, František FORET and Petr KUBÁŇ

Basic information

Original name

SENSITIVE DETERMINATION OF GLUTATHIONE IN VARIOUS BIOLOGICAL SAMPLES BY CAPILLARY ELECTROPHORESIS WITH GREEN LASER INDUCED FLUORESCENCE DETECTION

Authors

LAČNÁ, Júlia (703 Slovakia, belonging to the institution), Jan PREISLER (203 Czech Republic, belonging to the institution), František FORET (203 Czech Republic, belonging to the institution) and Petr KUBÁŇ (203 Czech Republic, belonging to the institution)

Edition

BRNO, CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS, p. 232-236, 5 pp. 2014

Publisher

INST ANALYTICAL CHEMISTRY ASCR, V V I-IAC

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

printed version "print"

RIV identification code

RIV/00216224:14310/14:00083392

Organization unit

Faculty of Science

ISBN

978-80-904959-2-0

UT WoS

000354547400077

Keywords in English

GLUTATHIONE; fluorescence

Abstract

V originále

Laser induced fluorescence capillary electrophoresis (CE-LIF) with 515 nm green laser module was used for sensitive determination of glutathione (GSH) in various biological samples. For the first time, eosin-5-maleimide was used as a fluorescent tag in CE. Studies were performed to optimize the derivatization conditions (the ratio of reagent to analyte, the reaction time, temperature, etc.). CE-LIF also offers a unique opportunity to monitor the stability of the reagent and various reaction products. We demonstrate the applicability of the developed method on determination of GSH in real biological samples (exhaled breath condensate and saliva) with LODs as low as 180 pM.

Citovat

LAČNÁ, Júlia, Jan PREISLER, František FORET and Petr KUBÁŇ. SENSITIVE DETERMINATION OF GLUTATHIONE IN VARIOUS BIOLOGICAL SAMPLES BY CAPILLARY ELECTROPHORESIS WITH GREEN LASER INDUCED FLUORESCENCE DETECTION. In Foret, F Krenkova, J Drobnikova, I Guttman, A Kleparnik, K. CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS. BRNO: INST ANALYTICAL CHEMISTRY ASCR, V V I-IAC, 2014, p. 232-236. ISBN 978-80-904959-2-0.

@inproceedings{1306293,
   author = {Lačná, Júlia and Preisler, Jan and Foret, František and Kubáň, Petr},
   address = {BRNO},
   booktitle = {CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS},
   editor = {Foret, F Krenkova, J Drobnikova, I Guttman, A Kleparnik, K},
   keywords = {GLUTATHIONE; fluorescence},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {BRNO},
   isbn = {978-80-904959-2-0},
   pages = {232-236},
   publisher = {INST ANALYTICAL CHEMISTRY ASCR, V V I-IAC},
   title = {SENSITIVE DETERMINATION OF GLUTATHIONE IN VARIOUS BIOLOGICAL SAMPLES BY CAPILLARY ELECTROPHORESIS WITH GREEN LASER INDUCED FLUORESCENCE DETECTION},
   year = {2014}
}

TY  - JOUR
ID  - 1306293
AU  - Lačná, Júlia - Preisler, Jan - Foret, František - Kubáň, Petr
PY  - 2014
TI  - SENSITIVE DETERMINATION OF GLUTATHIONE IN VARIOUS BIOLOGICAL SAMPLES BY CAPILLARY ELECTROPHORESIS WITH GREEN LASER INDUCED FLUORESCENCE DETECTION
PB  - INST ANALYTICAL CHEMISTRY ASCR, V V I-IAC
CY  - BRNO
SN  - 9788090495920
KW  - GLUTATHIONE
KW  - fluorescence
N2  - Laser induced fluorescence capillary electrophoresis (CE-LIF) with 515 nm green laser module was used for sensitive determination of glutathione (GSH) in various biological samples. For the first time, eosin-5-maleimide was used as a fluorescent tag in CE. Studies were performed to optimize the derivatization conditions (the ratio of reagent to analyte, the reaction time, temperature, etc.). CE-LIF also offers a unique opportunity to monitor the stability of the reagent and various reaction products. We demonstrate the applicability of the developed method on determination of GSH in real biological samples (exhaled breath condensate and saliva) with LODs as low as 180 pM.
ER  -

LAČNÁ, Júlia, Jan PREISLER, František FORET and Petr KUBÁŇ. SENSITIVE DETERMINATION OF GLUTATHIONE IN VARIOUS BIOLOGICAL SAMPLES BY CAPILLARY ELECTROPHORESIS WITH GREEN LASER INDUCED FLUORESCENCE DETECTION. In Foret, F Krenkova, J Drobnikova, I Guttman, A Kleparnik, K. \textit{CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS}. BRNO: INST ANALYTICAL CHEMISTRY ASCR, V V I-IAC, 2014, p.~232-236. ISBN~978-80-904959-2-0.

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