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@proceedings{1306852,
author = {Dvořáková, Hana and Jedličková, Lucie and Kašný, Martin and Brož, Petr and Ilgová, Jana and Gelnar, Milan and Mikeš, Libor},
booktitle = {22nd Helminthological Days},
keywords = {next-generation sequencing; Monogenea; transcriptome},
language = {eng},
isbn = {978-80-7444-032-8},
title = {Transcriptomic analyses of a monogenean: Next-Generation Sequencing techniques in hands of parasitologists.},
year = {2015}
}
TY - CONF
ID - 1306852
AU - Dvořáková, Hana - Jedličková, Lucie - Kašný, Martin - Brož, Petr - Ilgová, Jana - Gelnar, Milan - Mikeš, Libor
PY - 2015
TI - Transcriptomic analyses of a monogenean: Next-Generation Sequencing techniques in hands of parasitologists.
SN - 9788074440328
KW - next-generation sequencing
KW - Monogenea
KW - transcriptome
N2 - Next-generation sequencing methods currently represent a robust tool enabling significant enrichment of our knowledge of biological systems at a level which has not been possible ever before. Unfortunately, the transcriptomic/genomic data on members of Monogenea are still extremely limited. Therefore we adopted the NGS techniques to generate a first high quality transcriptome of a representative of blood-feeding monogeneans - Eudiplozoon nipponicum (Diplozoidae: Heteronchoinea), the ectoparasite from the gills of common carp Cyprinus carpio. Using 454 sequencing technology (GS FLX System, Roche), a total of 324 941 reads were generated (average length 423 bp) and assembled into 6910 contigs. The second transcriptome analysis was performed by the MiSeq System (Illumina). A total of 74 823 255 input read pairs, a total of 142 813 all transcripts (median contig length 367 bp) and a total of 119 136 of genes (median contig length 334 bp) were obtained. Currently, the comprehensive bioinformatic analysis is running - the assemblies are in the process of evaluation and a pipeline for annotation of particular genes is being developed. Special attention was paid to peptidases that could be involved in digestion of host´s blood and other biological processes in E. nipponicum.
ER -
DVOŘÁKOVÁ, Hana, Lucie JEDLIČKOVÁ, Martin KAŠNÝ, Petr BROŽ, Jana ILGOVÁ, Milan GELNAR and Libor MIKEŠ. Transcriptomic analyses of a monogenean: Next-Generation Sequencing techniques in hands of parasitologists. In \textit{22nd Helminthological Days}. 2015. ISBN~978-80-7444-032-8.
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