a 2015

Structure and specificity of lectin from bacterium Burkholderia pseudomallei

SÝKOROVÁ, Petra, Jitka NOVOTNÁ, Gabriel DEMO, Eva DUBSKÁ, Jan KOMÁREK et. al.

Basic information

Original name

Structure and specificity of lectin from bacterium Burkholderia pseudomallei

Authors

SÝKOROVÁ, Petra (203 Czech Republic, belonging to the institution), Jitka NOVOTNÁ (203 Czech Republic, belonging to the institution), Gabriel DEMO (703 Slovakia, belonging to the institution), Eva DUBSKÁ (203 Czech Republic, belonging to the institution), Jan KOMÁREK (203 Czech Republic, belonging to the institution), Martina POKORNÁ (203 Czech Republic, belonging to the institution) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

2015

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/15:00080927

Organization unit

Faculty of Science

Keywords (in Czech)

lektiny; Burkholderia pseudomallei

Keywords in English

lectins; Burkholderia pseudomallei
Změněno: 28/4/2016 14:40, Ing. Andrea Mikešková

Abstract

V originále

A new lectin from B. pseudomallei was identified, which did not show any significant sequence similarity to the known proteins. The gene coding the lectin was cloned and protein expression and purification were successfully optimized. Surface plasmon resonance (SPR) and titration microcalorimetry (ITC) were used to characterize the interactions between the lectin and saccharides. Both methods revealed the lectin ability to bind D-mannose and mannosylated derivatives. The structure of the protein was solved using X-ray diffraction and showed a novel fold of bacterial lectins. The lectin is present as a monomer in solution as determined using analytical ultracentrifugation, which was also confirmed in the crystal structure. According to its structure and sequence, the protein belongs to a not yet described family of lectins.

Links

ED1.1.00/02.0068, research and development project
Name: CEITEC - central european institute of technology
GA13-25401S, research and development project
Name: Studium proteinů z patogenů zapojených do rozpoznávání hostitelského organismu
Investor: Czech Science Foundation