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@proceedings{1306888,
author = {Sýkorová, Petra and Novotná, Jitka and Demo, Gabriel and Dubská, Eva and Komárek, Jan and Pokorná, Martina and Wimmerová, Michaela},
keywords = {lectins; Burkholderia pseudomallei},
language = {eng},
title = {Structure and specificity of lectin from bacterium Burkholderia pseudomallei},
year = {2015}
}
TY - CONF
ID - 1306888
AU - Sýkorová, Petra - Novotná, Jitka - Demo, Gabriel - Dubská, Eva - Komárek, Jan - Pokorná, Martina - Wimmerová, Michaela
PY - 2015
TI - Structure and specificity of lectin from bacterium Burkholderia pseudomallei
KW - lectins
KW - Burkholderia pseudomallei
N2 - A new lectin from B. pseudomallei was identified, which did not show any significant sequence similarity to the known proteins. The gene coding the lectin was cloned and protein expression and purification were successfully optimized. Surface plasmon resonance (SPR) and titration microcalorimetry (ITC) were used to characterize the interactions between the lectin and saccharides. Both methods revealed the lectin ability to bind D-mannose and mannosylated derivatives. The structure of the protein was solved using X-ray diffraction and showed a novel fold of bacterial lectins. The lectin is present as a monomer in solution as determined using analytical ultracentrifugation, which was also confirmed in the crystal structure. According to its structure and sequence, the protein belongs to a not yet described family of lectins.
ER -
SÝKOROVÁ, Petra, Jitka NOVOTNÁ, Gabriel DEMO, Eva DUBSKÁ, Jan KOMÁREK, Martina POKORNÁ and Michaela WIMMEROVÁ. \textit{Structure and specificity of lectin from bacterium Burkholderia pseudomallei}. 2015.
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