J 2015

Monitoring the ionic content of exhaled breath condensate in various respiratory diseases by capillary electrophoresis with contactless conductivity detection

GREGUŠ, Michal, František FORET, Dagmar KINDLOVÁ, Eva POKOJOVÁ, Marek PLUTINSKÝ et. al.

Basic information

Original name

Monitoring the ionic content of exhaled breath condensate in various respiratory diseases by capillary electrophoresis with contactless conductivity detection

Authors

GREGUŠ, Michal (703 Slovakia, belonging to the institution), František FORET (203 Czech Republic, belonging to the institution), Dagmar KINDLOVÁ (203 Czech Republic), Eva POKOJOVÁ (203 Czech Republic), Marek PLUTINSKÝ (703 Slovakia, belonging to the institution), Martina DOUBKOVÁ (203 Czech Republic, belonging to the institution), Zdeněk MERTA (203 Czech Republic, belonging to the institution), Ilona BINKOVÁ (203 Czech Republic), Jana SKŘIČKOVÁ (203 Czech Republic, belonging to the institution) and Petr KUBÁŇ (203 Czech Republic, guarantor, belonging to the institution)

Edition

JOURNAL OF BREATH RESEARCH, BRISTOL, IOP PUBLISHING LTD, 2015, 1752-7155

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 4.177

RIV identification code

RIV/00216224:14740/15:00080937

Organization unit

Central European Institute of Technology

DOI

UT WoS

000355656200015

Keywords in English

exhaled breath condensate; capillary electrophoresis; contactless conductivity detection; anions and cations; respiratory diseases

Tags

Tags

International impact, Reviewed
Změněno: 26/4/2016 11:45, Olga Křížová

Abstract

V originále

The analysis of an ionic profile of exhaled breath condensate (EBC) by capillary electrophoresis with contactless conductivity detection and double opposite end injection, is demonstrated. A miniature sampler made from a 2 ml syringe and an aluminium cooling cylinder was used for the fast collection of EBC (under one minute). Analysis of the collected EBC was performed in a 60 mM 2-(N-morpholino) ethanesulfonic acid, 60 mM L-histidine background electrolyte with 30 mu M cetyltrimethylammonium bromide and 2 mM 18-crown-6 at pH 6, and excellent repeatability of migration times (RSD < 1.3% (n = 7)) and peak areas (RSD < 7% (n = 7)) of 14 ions (inorganic anions, cations and organic acids) was obtained. It is demonstrated that the analysis of EBC samples obtained from patients with various respiratory diseases (chronic obstructive pulmonary disease, asthma, pulmonary fibrosis, sarcoidosis, cystic fibrosis) is possible in less than five minutes and the ionic profile can be compared with the group of healthy individuals. The analysis of the ionic profile of EBC samples provides a set of data in which statistically significant differences among the groups of patients could be observed for several clinically relevant anions (nitrite, nitrate, acetate, lactate). The developed collection system and method provides a highly reproducible and fast way of collecting and analyzing EBC, with future applicability in point-of-care diagnostics.

Links

ED1.1.00/02.0068, research and development project
Name: CEITEC - central european institute of technology
GA13-21919S, research and development project
Name: Vývoj neinvazivních metod odběru vzorků a analýzy v point-of-care dignostice biologických materiálů.
Investor: Czech Science Foundation

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