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@article{1307054,
author = {Greguš, Michal and Foret, František and Kindlová, Dagmar and Pokojová, Eva and Plutinský, Marek and Doubková, Martina and Merta, Zdeněk and Binková, Ilona and Skřičková, Jana and Kubáň, Petr},
article_location = {BRISTOL},
article_number = {2},
doi = {http://dx.doi.org/10.1088/1752-7155/9/2/027107},
keywords = {exhaled breath condensate; capillary electrophoresis; contactless conductivity detection; anions and cations; respiratory diseases},
language = {eng},
issn = {1752-7155},
journal = {JOURNAL OF BREATH RESEARCH},
title = {Monitoring the ionic content of exhaled breath condensate in various respiratory diseases by capillary electrophoresis with contactless conductivity detection},
url = {http://iopscience.iop.org/article/10.1088/1752-7155/9/2/027107/meta},
volume = {9},
year = {2015}
}
TY - JOUR
ID - 1307054
AU - Greguš, Michal - Foret, František - Kindlová, Dagmar - Pokojová, Eva - Plutinský, Marek - Doubková, Martina - Merta, Zdeněk - Binková, Ilona - Skřičková, Jana - Kubáň, Petr
PY - 2015
TI - Monitoring the ionic content of exhaled breath condensate in various respiratory diseases by capillary electrophoresis with contactless conductivity detection
JF - JOURNAL OF BREATH RESEARCH
VL - 9
IS - 2
SP - nestránkováno
EP - nestránkováno
PB - IOP PUBLISHING LTD
SN - 17527155
KW - exhaled breath condensate
KW - capillary electrophoresis
KW - contactless conductivity detection
KW - anions and cations
KW - respiratory diseases
UR - http://iopscience.iop.org/article/10.1088/1752-7155/9/2/027107/meta
L2 - http://iopscience.iop.org/article/10.1088/1752-7155/9/2/027107/meta
N2 - The analysis of an ionic profile of exhaled breath condensate (EBC) by capillary electrophoresis with contactless conductivity detection and double opposite end injection, is demonstrated. A miniature sampler made from a 2 ml syringe and an aluminium cooling cylinder was used for the fast collection of EBC (under one minute). Analysis of the collected EBC was performed in a 60 mM 2-(N-morpholino) ethanesulfonic acid, 60 mM L-histidine background electrolyte with 30 mu M cetyltrimethylammonium bromide and 2 mM 18-crown-6 at pH 6, and excellent repeatability of migration times (RSD < 1.3% (n = 7)) and peak areas (RSD < 7% (n = 7)) of 14 ions (inorganic anions, cations and organic acids) was obtained. It is demonstrated that the analysis of EBC samples obtained from patients with various respiratory diseases (chronic obstructive pulmonary disease, asthma, pulmonary fibrosis, sarcoidosis, cystic fibrosis) is possible in less than five minutes and the ionic profile can be compared with the group of healthy individuals. The analysis of the ionic profile of EBC samples provides a set of data in which statistically significant differences among the groups of patients could be observed for several clinically relevant anions (nitrite, nitrate, acetate, lactate). The developed collection system and method provides a highly reproducible and fast way of collecting and analyzing EBC, with future applicability in point-of-care diagnostics.
ER -
GREGUŠ, Michal, František FORET, Dagmar KINDLOVÁ, Eva POKOJOVÁ, Marek PLUTINSKÝ, Martina DOUBKOVÁ, Zdeněk MERTA, Ilona BINKOVÁ, Jana SKŘIČKOVÁ and Petr KUBÁŇ. Monitoring the ionic content of exhaled breath condensate in various respiratory diseases by capillary electrophoresis with contactless conductivity detection. \textit{JOURNAL OF BREATH RESEARCH}. BRISTOL: IOP PUBLISHING LTD, 2015, vol.~9, No~2, p.~nestránkováno, 10 pp. ISSN~1752-7155. Available from: https://dx.doi.org/10.1088/1752-7155/9/2/027107.
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